Thermo Scientific™ Pierce™ Chicken IgY Purification Kit

Description

Thermo Scientific™ Pierce Chicken IgY Purification Kit purifies chicken antibodies from hen egg yolks using a unique delipidation reagent to separate protein from lipid and a precipitation reagent to selectively isolate the IgY fraction.

The IgY Purification Kit is simple and easy to use. After isolating a yolk using the supplied egg separator, lipids are effectively removed by incubation and centrifugation in Pierce Delipidation Reagent. This first reagent is also effective as a storage solution, enabling multiple yolks to be collected and stored for simultaneous extraction at a later date. Upon addition of the specialized Pierce IgY Precipitation Reagent and subsequent centrifugation, a nearly pure pellet of intact IgY results; it is easily dissolved in phosphate buffered saline (PBS) or other storage buffer. The purified IgY is ready for use in immunodetection methods or antigen-specific purification without interference from lipids.

Highlights:

• Cost-effective – purifies twice as much IgY per egg than other popular kits, providing a lower cost-per-milligram of purified IgY

• High purity – method yields chicken antibody that is 85 to 95% pure (based on SDS-PAGE analysis)

• Easy to use – simple precipitation method purifies chicken IgY without affinity columns

• Flexible protocol – eggs can be harvested and quickly stored in buffer for purification at a later date

• Convenient – use eggs directly out of refrigerator; no need to equilibrate them to room temperature

Includes:

Egg separator, delipidation reagent and IgY precipitation reagent