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  6. Thermo Scientific™ Pierce™ Chicken IgY Purification Kit

Thermo Scientific™ Pierce™ Chicken IgY Purification Kit

Catalog No. PI44922
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PI44922 5 L
PI44918 1 L
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Catalog No. PI44922 Supplier Thermo Scientific™ Supplier No. 44922
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Description

Purify chicken antibodies from hen egg yolks with a unique delipidation reagent and precipitation reagent to selectively isolate the IgY fraction.

Thermo Scientific™ Pierce Chicken IgY Purification Kit purifies chicken antibodies from hen egg yolks using a unique delipidation reagent to separate protein from lipid and a precipitation reagent to selectively isolate the IgY fraction.

The IgY Purification Kit is simple and easy to use. After isolating a yolk using the supplied egg separator, lipids are effectively removed by incubation and centrifugation in Pierce Delipidation Reagent. This first reagent is also effective as a storage solution, enabling multiple yolks to be collected and stored for simultaneous extraction at a later date. Upon addition of the specialized Pierce IgY Precipitation Reagent and subsequent centrifugation, a nearly pure pellet of intact IgY results; it is easily dissolved in phosphate buffered saline (PBS) or other storage buffer. The purified IgY is ready for use in immunodetection methods or antigen-specific purification without interference from lipids.

Highlights:

  • Cost-effective – purifies twice as much IgY per egg than other popular kits, providing a lower cost-per-milligram of purified IgY

  • High purity – method yields chicken antibody that is 85 to 95% pure (based on SDS-PAGE analysis)

  • Easy to use – simple precipitation method purifies chicken IgY without affinity columns

  • Flexible protocol – eggs can be harvested and quickly stored in buffer for purification at a later date

  • Convenient – use eggs directly out of refrigerator; no need to equilibrate them to room temperature

Includes:

Egg separator, delipidation reagent and IgY precipitation reagent

Specifications

Product Type IgY Purification Kit
Product Line Pierce
Column Type Precipitation
Format Kit, Bottle
Purification Target IgY Antibody
Content And Storage Sufficient For: Purifying chicken IgY from 25 egg yolks
• Delipidation Reagent, 5 x 500 mL
• IgY Precipitation Reagent, 5 x 500 mL
• Egg Separator, 5 separators

Store at 4°C.
Quantity 5 L

Frequently Asked Questions (FAQs)

Is there any difference between IgY from serum versus egg yolks?

No. Serum and egg IgY are the same. The advantage of collecting IgY from the yolks is the high concentration of IgY per yolk versus IgY in serum.
Can I immobilize IgY on Protein A, Protein G or Protein A/G to immunoprecipitate or affinity purify the antigen?

No. IgY does not bind to Protein A, Protein G or Protein A/G; however, AminoLink Plus Resin (Cat. No. 20501) or Kit (Cat. No. 44894) can immobilize IgY. The AminoLink Plus Resin is aldehyde-activated and reacts with primary amines on the IgY and forms a covalent linkage.
What is the molecular weight of IgY?

IgY has a mass of 180kDa, which is more than IgG because the IgY heavy chain has an extra domain on the Fc region. The light chains are ~22 kDa and the heavy chains are ~68 kDa; however, estimates vary in the literature.
How should I store the IgY purified using the Thermo Scientific Chicken IgY Purification Kit??

Store the purified IgY at 0-4°C (< 6 months). For longer storage, separate the IgY into small volumes, add a small amount of glycerol (< 10%) to help stabilize the IgY and store at -20°C.
How much IgY can be purified with the Thermo Scientific Chicken IgY Purification Kit?

The kits can purify up to 100 mg of chicken IgY per yolk (i.e., 500 mg with Cat. No. 44918 and 2,500 mg with Cat. No. 44922).
Why do I need to use cold eggs for IgY purification using the Thermo Scientific Chicken IgY Purification Kit?

Delipidation occurs optimally at 4°C, so it is best to have eggs and reagents cold.
Do I need to change the protocol when using frozen eggs for IgY purification using the Thermo Scientific Chicken IgY Purification Kit?

To store yolks frozen before purification, mix them well with cold Delipidation Reagent (two times the original yolk volume), and store them for approximately one year.
Do I need to use fresh yolks for IgY purification using the Thermo Scientific Chicken IgY Purification Kit?

Eggs stored in the refrigerator for up to two months may be used or yolks can be processed and stored frozen.

For Research Use Only. Not for use in diagnostic procedures.