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Thermo Scientific™ Pierce™ Bovine Serum Albumin Standard Ampules, 2 mg/mL

Catalog No. PI23209
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PI23209 10 x 1 mL
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Catalog No. PI23209 Supplier Thermo Scientific™ Supplier No. 23209
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Description

High-quality, calibrated BSA solutions (2 mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration.

Thermo Scientific™ Pierce BSA Protein Assay Standards are high-quality reference samples for generating accurate standard curves and calibration controls in total protein assays.

These bovine serum albumin (BSA) solutions are protein concentration reference standards for use in BCA, Bradford and other protein assay protocols. BSA is the universally accepted reference protein for total protein quantitation. The albumin standard is precisely formulated at 2mg/mL in an ultrapure 0.9% sodium chloride (saline) solution. The concentration of the stock solution is calibrated by direct comparison to purified BSA (Fraction V) from the National Institute of Standards and Technology (NIST™). The product is available in three convenient package formats: 1mL glass ampules, 50mL polypropylene bottles, and complete sets containing seven ready-to-use dilutions.

Highlights:
• Universal – recognized as the industry standard for protein quantitation in colorimetric protein assays
• Pure and stable – supplied in ultrapure 0.9% saline solution with 0.05% sodium azide; room temperature stable
• Accurate and consistent – precisely formulated at 2.00 +/-0.03mg/mL compared to a NIST reference
• Convenient – choose 1mL ampules, a 50mL bottle, or a complete set containing seven ready-to-use dilutions

Includes:
2 mg/mL BSA solution (Cat. No. 23209, 23210) or 7-solution set of different concentrations (Cat. No. 23208)

Recommended for:
Protein assay quantitation standard; Protein recovery control for desalting and other column procedures; General calibration of spectrophotometer UV-lamp (A280)

Specifications

Content And Storage Store at room temperature.
Description Pierce Bovine Serum Albumin Standard Ampules, 2mg/mL
Assay Protein Standards
Product Line Pierce
Product Type Protein Quantitation Assay
Specificity Not Target-Specific
For Use With (Application) Internal Standard
Quantity 10 x 1 mL

Frequently Asked Questions (FAQs)

Can you provide the shelf-life for the Pierce Bovine Serum Albumin Standard, 2 mg/mL?

The Pierce Bovine Serum Albumin Standard, 2 mg/mL is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
Can you provide the shelf life for Pierce Bovine Serum Albumin Standard Ampules, 2 mg/mL?

Pierce Bovine Serum Albumin Standard Ampules, 2 mg/mL are covered under our general 1-year warranty and are guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (room temperature). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

When using a NanoDrop spectrophotometer, what is the difference between controls versus standards?

NanoDrop CF-1 Calibration Fluid is the only photometric standard available to assess the accuracy of the NanoDrop spectrophotometer pedestal. If the control sample shows unexpected results the user should confirm the calibration of the instrument with the CF-1 fluid.
Some control examples include:
- Nucleic Acids: Fermentas GeneRuler Express DNA Ladder 100-5000 bp (Cat. No. SM1551)
- Protein: Pierce Bovine Serum Albumin Standard Ampules, 2 mg/mL (Cat. No. 23209)

How should I open the glass BSA standard ampules?

There are several ampule-breaking devices sold through various suppliers such as Fisher Scientific (see https://www.fishersci.com/us/en/catalog/search/products?keyword=ampule+breaker&nav=) that can be used.
Can I re-use diluted BSA standards for subsequent protein assays?

The challenge of diluted BSA standards is that they need to be protected from microbial growth, evaporation and protein precipitation. These will result in artificially high or low protein concentration values. Including additional sodium azide with the diluted protein standards to maintain a final concentration of 0.05% will help prevent microbial growth. The shelf life of diluted standards can be extended if they are sealed and kept refrigerated.

How are the BSA standards tested to make sure they are at a concentration of 2 mg/mL?

The BSA standards are tested by measuring their absorbance at 280 nm and the results are compared to a 2 mg/mL standard that we obtained from the National Institute of Standards and Technology (NIST). The official standard is of traceable origin with an identification number of NIST # 927. Our specifications require the BSA concentration to be 2.00 ± 0.03 mg/mL.
What are BSA standards?

BSA standards are preformulated solutions of Bovine Albumin Fraction V in 0.9% NaCl. The standards also include 0.05% sodium azide to prevent microbial contamination. The BSA standards can be diluted to predetermined concentrations in order to prepare standard curves for protein assays such as BCA Protein Assay Kit (Cat. No. 23225), 660 nm Protein Assay Reagent (Cat. No. 22660), Coomassie Plus (Bradford) Assay Kit (Cat. No. 23236) and Modified Lowry Protein Assay Kit (Cat. No. 23240). The standards are prepared at a concentration of 2 mg/mL. They can be stored at room temperature until opened, and then should be refrigerated after opening.
What should the BSA standard be diluted in to create a standard curve?

The BSA standard should be treated the same as the protein of interest and should be diltuted in the same buffer as the protein of interest. That way, if the buffer itself causes some response to the protein assay, it will be accounted for.

For Research Use Only. Not for use in diagnostic procedures.