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Thermo Scientific™ Pierce™ Binding and Elution Buffers for IgG Purification

Ensure high-yield, nondenaturing antibody purification using IgG affinity purification supports with these gentle buffer solutions.

$69.56 - $520.80

Specifications

Target IgG
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Products
Catalog Number Mfr. No. Description Quantity Price Quantity    

PI21001

 
thermo scientific™
PI21001
Pierce Protein A IgG Binding Buffer 1L Each for $135.45

PI21004

 
thermo scientific™
PI21004
Pierce IgG Elution Buffer 1L Each for $128.10

PI21007

 
thermo scientific™
PI21007
Pierce Protein A IgG Binding Buffer 3.75L Each for $381.15

PI21009

 
thermo scientific™
PI21009
Pierce IgG Elution Buffer 3.75L Each for $317.10

PI21011

 
thermo scientific™
PI21011
Pierce Protein G IgG Binding Buffer 3.75L Each for $394.80

PI21012

 
thermo scientific™
PI21012
Pierce Gentle Ag/Ab Binding Buffer, pH 8.0 3.75L Each for $368.55

PI21013

 
thermo scientific™
PI21013
Pierce Gentle Ag/Ab Elution Buffer, pH 6.6 3.75L Each for $520.80

PI21019

 
thermo scientific™
21019
Pierce Protein G IgG Binding Buffer 1L Each for $124.95

PI21027

 
thermo scientific™
PI21027
Pierce Gentle Ag/Ab Elution Buffer, pH 6.6 500mL Each for $178.50

PI54200

 
thermo scientific™
PI54200
Pierce Protein A/G IgG Binding Buffer 240mL Each for $69.56

PI21020

 
thermo scientific™
PI21020
Pierce Gentle Ag/Ab Binding Buffer, pH 8.0 1L Each for $191.10

PI21028

 
thermo scientific™
21028
Pierce IgG Elution Buffer, pH 2.0 1L Each for $109.20

PI21030

 
thermo scientific™
PI21030
Pierce Gentle Ag/Ab Binding and Elution Buffer Kit 200mL kit Each for $142.80
Description & Specifications

Specifications

Target IgG

Thermo Scientific™ Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.

These products include IgG Binding Buffers specific for each immunoglobulin-binding protein (Protein A, Protein G, Protein A/G). The Pierce IgG Elution Buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins, including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems.

The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.

Finally, the Mouse IgG1 Mild Elution Buffer, when used in combination with Protein A resin, enables selective elution and separation of the IgG1 subclass from total bound mouse IgG.

Highlights:

  • Three optimized binding buffers – for Protein A, Protein G or Protein A/G

  • Two elution buffer options – acidic (pH 2.8) or high salt (near-neutral)

  • Special system for mouse IgG1 – selective, two-stage elution from Protein A agarose

Item-Specific Features:

  • Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA

  • Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA

  • Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide

  • IgG Elution Buffer: pH 2.8; amine-based

  • Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide

  • Gentle Ag/Ab Elution Buffer: pH 6.6; high salt (ionic strength)

  • Mouse IgG1 Mild Elution Buffer: pH 6.0; contains 0.02% sodium azide

Includes:

Kits contain both binding and elution buffers