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Thermo Scientific™ Pierce™ Microplate BCA Protein Assay Kit - Reducing Agent Compatible

Catalog No. PI23252
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PI23252 275 mL
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Catalog No. PI23252 Supplier Thermo Scientific™ Supplier No. 23252
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Description

Reducing agent-compatible version of our popular BCA Reagent Kit to measure protein concentration in samples containing DTT or BME thiol-reductants.

This reducing agent-compatible (RAC) version of the Thermo Scientific™ Pierce BCA Protein Assay provides accurate determination of protein concentration in samples that contain dithiothreitol (DTT), 2-mercaptoethanol (BME), TCEP and other disulfide reducing agents.

The BCA Protein Assay Kit - Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA Assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the popular Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, 2-ME or TCEP. Available in tube and microplate formats, these reducing agent compatible (RAC) BCA Kits extend the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.

Highlights:

  • Compatible – assay samples that contain up to 5mM DTT, 35mM BME, or 10mM TCEP

  • BCA technology – only a slight modification of the standard BCA Protein Assay protocol (15-minute incubation with Compatibility Reagent); no precipitation steps required

  • Small samples – requires only 25μL (standard kit) or less than 10μL (microplate kit) of sample

  • Colorimetric – measure with a standard spectrophotometer or plate reader (562nm)

  • Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods

  • High linearity – linear working range for BSA equals 125 to 2000μg/mL

Includes:

BCA Reagents A and B, Compatibility Reagent, Reconstitution Buffer, and Albumin Standard (2mg/mL); Microplate kit also includes assay-compatible 96-well microplates

Specifications

Content And Storage Sufficient For: 980 microplate assays with 9 μL samples
• BCA Reagent A, 250 mL
• BCA Reagent B, 25 mL
• Compatibility Reagent, 48 x 10 mg
• Reconstitution Buffer, 15 mL
• Albumin Standard Ampules, 2mg/mL, 10 x 1 mL
• 96-Well Microplates, 20 plates

Store at room temperature
Description Pierce BCA Microplate Protein Assay Kit, Reducing Agent Compatible (RAC)
Assay BCA Assay
Detection Method Colorimetric
Product Line Pierce
Product Type Protein Quantitation Assay
Specificity Not Target-Specific
Sufficient For 980 Microplate Assays
For Use With (Application) Solution-based Detection, Absorbance
For Use With (Equipment) Spectrophotometer, Microplate Reader
Quantity 275 mL
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For Research Use Only. Not for use in diagnostic procedures.