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Thermo Scientific™ Pierce™ BCA™ Protein Assay, Reducing Agent Compatible (RAC)

Reducing agent-compatible version of our popular BCA Reagent Kit to measure protein concentration in samples containing DTT or BME thiol-reductants.

Manufacturer:  Thermo Scientific™ PI23250

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Catalog No. PI23250




This reducing agent-compatible (RAC) version of the Thermo Scientific™ Pierce BCA Protein Assay provides accurate determination of protein concentration in samples that contain dithiothreitol (DTT), 2-mercaptoethanol (BME), TCEP and other disulfide reducing agents.

The BCA Protein Assay Kit - Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA Assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the popular Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, 2-ME or TCEP. Available in tube and microplate formats, these reducing agent compatible (RAC) BCA Kits extend the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.


  • Compatible – assay samples that contain up to 5mM DTT, 35mM BME, or 10mM TCEP

  • BCA technology – only a slight modification of the standard BCA Protein Assay protocol (15-minute incubation with Compatibility Reagent); no precipitation steps required

  • Small samples – requires only 25μL (standard kit) or less than 10μL (microplate kit) of sample

  • Colorimetric – measure with a standard spectrophotometer or plate reader (562nm)

  • Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods

  • High linearity – linear working range for BSA equals 125 to 2000μg/mL


BCA Reagents A and B, Compatibility Reagent, Reconstitution Buffer, and Albumin Standard (2mg/mL); Microplate kit also includes assay-compatible 96-well microplates

Description & Specifications


Albumin Standard Ampules, 2mg/mL, 10 x 1mL
Five-component kit with BCA reagents and standard
250 tube assays
Pierce BCA Tube Protein Assay Kit, Reducing Agent Compatible (RAC)
225mL kit


  • Buy up to $1,000 in protein gels and electrophoresis products and get an equal amount at no cost. This is an NLSU exclusive offer. You must apply and qualify for the program to be eligible.