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Thermo Scientific™ Pierce™ BCA Protein Assay Reagent A
Description
Thermo Scientific™ Pierce BCA Protein Assay Kit remains one of the most popular protein quantitation methods worldwide.
Additional Information and Online Ordering
- A component of the Pierce BCA Protein Assay Kit
- Kit is a two-component, high-precision, detergent-compatible assay that is used for total protein concentration determination compared to a protein standard
- Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research
- The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications
- Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy
- Estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm
- Exhibits less protein-to-protein variation than dye-binding methods (Bradford)
- Unaffected by typical concentrations of most ionic and non-ionic detergents
- Assay has a 30 min. incubation; much easier and four times faster than classical Lowry methods
- Linear working range for BSA of 20 to 2000 μg/mL
- Detect down to 5 μg/mL with the enhanced protocol
- Store at room temperature
Specifications
Specifications
| Content And Storage | Store at room temperature. |
| Description | Pierce BCA Protein Assay Reagent A |
| Assay | BCA Assay |
| Detection Method | Colorimetric |
| Product Line | Pierce |
| Product Type | Protein Quantitation Assay |
| Specificity | Not Target-Specific |
| For Use With (Application) | Solution-based Detection, Absorbance |
| For Use With (Equipment) | Spectrophotometer, Microplate Reader |
| Quantity | 500 mL |
For Research Use Only. Not for use in diagnostic procedures.