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Thermo Scientific™ Ionic Detergent Compatibility Reagent for Pierce™ 660nm Protein Assay Reagent

Catalog No. PI22663
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Catalog No. PI22663 Supplier Thermo Scientific™ Supplier No. 22663
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Description

Red-to-green (A660nm), ready-to-use, detergent- and reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.

Thermo Scientific™ Pierce 660nm Protein Assay is a quick, ready- to-use colorimetric method for total protein quantitation.

Additional Information and Online Ordering

The Pierce 660nm Protein Assay is a quick, ready-to-use colorimetric method for measuring protein concentration. The assay is more linear than coomassie-based Bradford assays and compatible with higher concentrations of most detergents, reducing agents and other commonly used reagents. The accessory Ionic Detergent Compatibility Reagent (IDCR) provides for even broader detergent compatibility, making the Pierce 660nm Protein Assay the only protein assay that is suitable for samples containing Laemmli SDS sample buffer with bromophenol blue.

Although the Pierce 660nm Protein Assay produces a higher level of protein-to-protein variation (37%) than other assays, such as the BCA Protein Assay, the simpler single-reagent format and broader substance compatibility make the 660nm Assay more convenient for many routine applications. The Pierce 660nm Protein Assay can be performed in either a test tube or microplate format.

The assay is based on a proprietary dye-metal complex that binds to proteins in an acidic medium. Upon binding, the reddish dye-metal complex turns to green, resulting in an absorbance shift that is measurable at wavelengths near 660nm (645 to 670). The dye interacts mainly with basic residues in proteins, such as histidine, arginine and lysine and to a lesser extent tyrosine, tryptophan and phenylalanine.

Highlights:

  • Versatile – works with a greater range of detergents and reducing agents than other dye-based assays

  • Fast – single reagent with a simple mix-and-read protocol; no working reagent to prepare

  • Accurate – produces standard curves that are more linear than with the Bradford assay method

  • Flexible – assay may be performed in test tubes or microplates

  • Conserve samples – requires only 10μL for microplate or 100μL for the test tube procedures

  • Convenient – room temperature storage means no waiting for reagent equilibration before use

Includes:

Kit contains Pierce 660nm Assay Reagent and a set of seven pre-diluted albumin protein assay standards

Specifications

Content And Storage Store between 2 to 25°C
Description Pierce Ionic Detergent Compatibility Reagent
Assay Ionic Detergent Compatibility Reagent
Detection Method Colorimetric
Product Line Pierce
Product Type Protein Quantitation Assay
Specificity Not Target-Specific
For Use With (Application) Solution-based Detection, Absorbance
For Use With (Equipment) Spectrophotometer, Microplate Reader
Quantity 5 x 1 g

Frequently Asked Questions (FAQs)

Can I use the Thermo Scientific 660 nm Protein Assay to quantify peptides?

The Thermo Scientific 660 nm Protein Assay can quantify peptides that are at least 2,500 Da if their compositions include amino acid residues that react with the dye-metal reagent (i.e., histidine, arginine, tyrosine, tryptophan and phenylalanine). For peptides smaller than 2,500 Da, use the Fluoraldehyde Reagent Solution (Cat. No. 26025), which detects amino groups.

Other peptide quantitation assays we offer are Thermo Scientific Pierce Quantitative Colorimetric Peptide Assay (Cat. No. 23275) and Thermo Scientific Pierce Quantitative Fluorometric Peptide Assay (Cat. No. 23290).

What is the linear range of the Thermo Scientific 660 nm Protein Assay?

The assay's linear range is 25-2,000 µg/mL using the test tube procedure and 50-2,000 µg/mL using the microplate procedure.

Can I measure the absorbance at a wavelength other than 660 nm when using the Thermo Scientific 660nm Protein Assay Kit?

If a 660nm filter is not available, the assay can be measured at wavelengths from 645 to 670nm; however, the assay linear range is 25-2,000 µg/mL and occurs only when the absorbance of the dye-protein complex is measured at 660 nm. Measuring the absorbance at wavelengths other than 660nm will result in a decrease of the assay's linear range and might increase the minimum detection level (i.e., decrease sensitivity).
What advantages does the Thermo Scientific 660nm Protein Assay have over other protein assays?

The Thermo Scientific 660 nm Protein Assay has the following advantages: • Room temperature stability of the assay reagent • A greater linear range than the coomassie-based Bradford assays • Compatibility with commonly used detergents and reducing agents • Compatibility with samples lysed in Laemmli sample buffer • Rapid mix-and-read protocol

What formats are available for the Thermo Scientific 660 nm Protein Assay?

We offer two formats: The complete kit (Cat. No. 22662) contains sufficient reagents for 300 test tube or 3,000 microplate assays and a set of pre-diluted BSA standards with concentrations from 125 to 2,000 µg/mL. The assay reagent (750 ml, Cat. No. 22660) is sufficient for 500 test tube or 5,000 microplate assays.

How does the Thermo Scientific 660nm Protein Assay Kit work?

The assay is based on a dye-metal complex that binds to proteins in an acidic solution. Upon binding, the reddish dye-metal complex changes to green, resulting in an absorbance shift measurable at 660 nm. The dye interacts mainly with basic residues, such as histidine, arginine and lysine and to a lesser extent, tyrosine, tryptophan and phenylalanine.
Can I mix Ionic Detergent Compatibility Reagent (Cat. No. 22663) with the Pierce 660nm Protein Assay Reagent (Cat. No. 22660) and store that solution at -20 degrees C in aliquots? If so, for how long?

We do not recommend storing the solution frozen.
Note: We also do not recommend storing it at 4 degrees C.

The user guide for the Pierce 660nm Protein Assay Reagent (Cat. No. 22660) states "The IDCR Solution is stable for 24 hours at room temperature." Do I need to discard all of the Ionic Detergent Compatibility Reagent (IDCR) (Cat. No. 22663) after 24 hours?

Once IDCR is dissolved in the Pierce 660nm Protein Assay Reagent, it can only be stored for one day at room temperature. Since you may not need to use the entire amount of IDCR within one day, you can calculate how much you need that day and store the remaining amount longer than 24 hours. IDCR is stable at room temperature if the pouch is closed very well and stored away from moisture. It can even be stored in a box with desiccant.

For Research Use Only. Not for use in diagnostic procedures.