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Applied Biosystems™ PicoPure™ RNA Isolation Kit

PicoPure™ RNA Isolation Kit

Supplier: Applied Biosystems™ KIT0214

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Catalog No. 36-100-7583

$3,025.48 / Each

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Description

The ARCTURUS™ PicoPure™ RNA Isolation Kit is designed to recover high-quality total RNA consistently from fewer than ten cells, even from a single cell. This kit is designed to provide consistent, efficient RNA recovery without sacrificing quality. Small volume elution allows you to maximize your recovery of RNA from small numbers of cells for use with your gene expression analysis.

Key Product Features:

Greater sensitivty—isolate RNA from even a single cell

Superior efficiency—maximize RNA recovery from samples

Streamlined concentration—elute with small volumes

Flexible application—compatible with many tube sizes

Integrated contamination prevention—column lids ensure that samples remain RNase free

Isolate RNA from Even a Single Cell
The PicoPure™ RNA Isolation Kit is engineered to recover high-quality total RNA consistently from fewer than ten cells. The high recovery rate attained using this kit is valuable not only for small samples containing picograms of RNA but also for samples containing up to 100μg of RNA. Since the RNA elutes in as little as 10μl of buffer, it is ready for gene expression analysis without any vacuum concentration. This kit is available in two sizes, enough for 40 or 200 isolations.

Get Superior RNA Recovery
Small, valuable samples often cannot be replaced. Performing gene expression studies on these precious samples requires getting maximum RNA recovery. Comparative studies show that, with small, RNA-poor cell or tissue samples, the PicoPure™ Kit dramatically outperforms other commercial total RNA isolation kits in efficiency.

Eliminate Time-consuming RNA Concentration
The simple isolation protocol consists of extracting cellular RNA, then loading the extract onto the specially designed MiraCol™ Purification Column to bind the RNA. After washing away impurities, the RNA elutes in only 10μl of buffer, ready for use without vacuum concentration or transfer into a new reaction tube.

High Yield Purification Columns
The PicoPure™ RNA Isolation Kit features unique, high-recovery MiraCol™ Purification Columns, engineered to maximize recovery from microscopic samples. The columns have convenient integrated lids to help ensure that samples remain RNase free. In addition, the Kit comes complete with all necessary buffers, an easy-to-follow User Guide, optimized protocols for handling Laser Capture Microdissected (LCM) samples, and protocols for isolating RNA from larger samples.

Deliver RNA Ready for Amplification and Microarray Analysis
RNA isolated with the PicoPure™ RNA Kit is ready, without vacuum concentration, for amplification with the RiboAmp™ RNA Amplification Kit. The two kits together allow for highly reproducible gene expression results.

Retain Low-abundance mRNA
The PicoPure™ RNA Isolation Kit preserves mRNA in all abundance classes through the isolation process, minimizing the loss of important genes of interest. Low, medium, and high-abundance genes are consistently recovered and available for expression analysis.

Maintain High RNA Quality
The PicoPure™ RNA isolation process allows for high-quality RNA. Total cellular RNA prepared from LCM samples remains intact after isolation, increasing confidence in subsequent molecular analysis.

Specifications

Content And Storage	Extraction buffer, purification reagents, purification columns, microcentrifuge tubes. Store at room temperature
Isolation Technology	Spin Column
Final Product Type	Total RNA
For Use With (Equipment)	ArcturusXT™ LCM Instrument
No. of Samples	200 samples
Quantity	200 samples
Target	Total RNA

Format	Spin Column
Sample Type	Laser Capture Microdissection (LCM), Tissue (Frozen)
High-throughput Compatibility	Not High-throughput Compatible (Manual)
For Use With (Application)	Microarray Analysis, Real-Time Quantitative PCR (qPCR)
Product Line	PicoPure™
Starting Material Amount	As little as a single cell.

Product Suggestions Documents

Documents

Scientific Resources

Application Notes

Application Notes & Tutorials: Isolation of Living In Vitro Cells Using the ArcturusXT™ Microdissection Instrument - Petri Dish Protocol File size: 3.3 MB

Application Notes & Tutorials: Microdissection to Microarrays: A Complete Solution for Gene Expression Profiling of Precious Tissue Samples File size: 1.2 MB

Application Notes & Tutorials: ArcturusXT™ Microdissection Systems: Optimized Protocol for Laser Microdissection of Living In Vitro Cells File size: 2.2 MB

Application Notes & Tutorials: From Postmortem Homogeneous Cells to Microarray Analysis: Optimized Protocol File size: 1.5 MB

Brochures

Brochures & Specifications: PicoPure® RNA Isolation Kit File size: 1.1 MB

Product Information

Manuals

UG: PicoPure RNA Isolation Kit Version: 1268200 Rev.D 06.2004 File size: 206.6 kB

PI: Arcturus Microgenomics Reagent Kit Version: R3592 Rev. D 03/2011 File size: 313.8 kB

User Bulletin: Arcturus CapSure LCM MicroCaps Version: MAN0014607 Rev.B.0 (14Feb2017) File size: 1.7 MB

Specification Sheets

Brochures & Specifications: ArcturusXT Laser Capture Microdissection System Product Brochure File size: 3.7 MB

For Research Use Only. Not for use in diagnostic procedures.