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Applied Biosystems™ PicoPure™ RNA Isolation Kit
Description
Key Product Features:
Isolate RNA from Even a Single Cell
The PicoPure™ RNA Isolation Kit is engineered to recover high-quality total RNA consistently from fewer than ten cells. The high recovery rate attained using this kit is valuable not only for small samples containing picograms of RNA but also for samples containing up to 100μg of RNA. Since the RNA elutes in as little as 10μl of buffer, it is ready for gene expression analysis without any vacuum concentration. This kit is available in two sizes, enough for 40 or 200 isolations.
Get Superior RNA Recovery
Small, valuable samples often cannot be replaced. Performing gene expression studies on these precious samples requires getting maximum RNA recovery. Comparative studies show that, with small, RNA-poor cell or tissue samples, the PicoPure™ Kit dramatically outperforms other commercial total RNA isolation kits in efficiency.
Eliminate Time-consuming RNA Concentration
The simple isolation protocol consists of extracting cellular RNA, then loading the extract onto the specially designed MiraCol™ Purification Column to bind the RNA. After washing away impurities, the RNA elutes in only 10μl of buffer, ready for use without vacuum concentration or transfer into a new reaction tube.
High Yield Purification Columns
The PicoPure™ RNA Isolation Kit features unique, high-recovery MiraCol™ Purification Columns, engineered to maximize recovery from microscopic samples. The columns have convenient integrated lids to help ensure that samples remain RNase free. In addition, the Kit comes complete with all necessary buffers, an easy-to-follow User Guide, optimized protocols for handling Laser Capture Microdissected (LCM) samples, and protocols for isolating RNA from larger samples.
Deliver RNA Ready for Amplification and Microarray Analysis
RNA isolated with the PicoPure™ RNA Kit is ready, without vacuum concentration, for amplification with the RiboAmp™ RNA Amplification Kit. The two kits together allow for highly reproducible gene expression results.
Retain Low-abundance mRNA
The PicoPure™ RNA Isolation Kit preserves mRNA in all abundance classes through the isolation process, minimizing the loss of important genes of interest. Low, medium, and high-abundance genes are consistently recovered and available for expression analysis.
Maintain High RNA Quality
The PicoPure™ RNA isolation process allows for high-quality RNA. Total cellular RNA prepared from LCM samples remains intact after isolation, increasing confidence in subsequent molecular analysis.
Specifications
Specifications
| Content And Storage | Extraction buffer, purification reagents, purification columns, microcentrifuge tubes. Store at room temperature |
| Format | Spin Column |
| Isolation Technology | Spin Column |
| Sample Type | Laser Capture Microdissection (LCM), Tissue (Frozen) |
| Final Product Type | Total RNA |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Equipment) | ArcturusXT™ LCM Instrument |
| For Use With (Application) | Microarray Analysis, Real-Time Quantitative PCR (qPCR) |
| No. of Samples | 200 samples |
| Product Line | PicoPure |
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Frequently Asked Questions (FAQs)
The PicoPure RNA Isolation Kit can be used with as little as a single cell to samples with up to 100 µg of RNA.
The PicoPure RNA Isolation Kit is not recommended for isolation of RNA smaller than 100 nucleotides.
Formalin-fixed paraffin-embedded (FFPE) samples introduce unique challenges for gene expression profiling and gene expression quantitation, including chemical modification and fragmentation of RNA molecules. The initial paraffin blot preparation and storage significant affect the RNA quality. Archival FFPE samples present additional challenges due to increased RNA degradation over time. As a result, not all FFPE samples contain high quality RNA.
In addition, a typical Bioanalyzer profile of RNA from an FFPE sample can look fragmented and degraded and as a result, that approach may not be useful in determining the transcribability of your RNA. Therefore, a tissue scrape test is recommended. You can use the entire adjacent section to isolate RNA and to access the RNA quantity and quality for intact RNA. Two sets of β-actin gene-specific primers designed to amplify short segments of DNA within the β-actin target sequence from the 5’ and 3’ of the β-actin gene can be used to estimate the amount of RNA in a given sample in relation to β-actin content using real-time PCR. The RNA quantity derived from the 3' primer set is used to quantitate the RNA in the FFPE tissue scrape. The ratio of the RNA yield obtained from both sets of PCR primers is the 3'⁄5' ratio and is used as an indication of RNA quality. If there is less product yield of the 5’ β-actin primers, it indicates that there is less full length of RNA.
The dissection and embedding steps are critical for the RNA quality for LCM samples. We suggest using the entire adjacent section or dissecting a large area of the section (>1000 cells) for RNA isolation. Before proceeding to LCM microdissection, we recommend assessing the quality of RNA isolated from the adjacent section. With good amount of RNA, one can assess the RNA quantity and quality using the Qubit RNA HS Assay Kit and the Agilent Bioanalyzer. This will help to make sure that good quality RNA is obtained from a low number of LCM cells.
The Arcturus PicoPure RNA Isolation Kit removes total cellular RNA from pico-scale samples including frozen sections, alcohol fixed-, or fresh samples. Please note, samples fixed with formaldehyde are not recommended for use with this kit.
For Research Use Only. Not for use in diagnostic procedures.