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Molecular Probes™ pHrodo™ Red, succinimidyl ester (pHrodo™ Red, SE) 
New pH-sensitive pHrodo™ Red dye conjugates give faster and more accurate results than any other phagocytosis assay.
Supplier: Molecular Probes™ P36600
Description
New pH-sensitive pHrodo™ Red dye conjugates give faster and more accurate results than any other phagocytosis assay
pHrodo™ Red dye conjugates are non-fluorescent outside the cell, but fluoresce brightly red in phagosomes
Get faster staining and more accurate results - without the need for wash steps or quencher dye
• Specific detection of phagocytosis and endocytosis
• Reduced signal variability and improved timing in sensitive experiments
• Multiplex with green dyes such as GFP, Fluo-4, or calcein
The fluorescence of the novel pHrodo™ Red dye dramatically increases as pH decreases from neutral to the acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes.
Use the ready-made pHrodo™ Red E.coli BioParticles™ conjugates in imaging or flow applications, or pHrodo™ Red SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.
Consult user Manual for solubility instructions.
Specifications
| 1 mg | |
| ∼650 | |
| Amine | |
| Active Ester, Succinimidyl Ester | |
| Classic Dyes | |
| pHrodo | |
| 560 nm |
| Succinimidyl Ester | |
| Store in freezer (-5°C to -30°C) and protect from light. | |
| Room Temperature | |
| pHrodo™ Red | |
| Red | |
| 587 nm |
For Research Use Only. Not for use in diagnostic procedures.