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Molecular Probes™ pHrodo™ Red, succinimidyl ester (pHrodo™ Red, SE)

Catalog No. P36600
Encompass
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Catalog No. P36600 Supplier Molecular Probes™ Supplier No. P36600
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New pH-sensitive pHrodo™ Red dye conjugates give faster and more accurate results than any other phagocytosis assay.

New pH-sensitive pHrodo™ Red dye conjugates give faster and more accurate results than any other phagocytosis assay

pHrodo™ Red dye conjugates are non-fluorescent outside the cell, but fluoresce brightly red in phagosomes

Get faster staining and more accurate results - without the need for wash steps or quencher dye

• Specific detection of phagocytosis and endocytosis
• Reduced signal variability and improved timing in sensitive experiments
• Multiplex with green dyes such as GFP, Fluo-4, or calcein

The fluorescence of the novel pHrodo™ Red dye dramatically increases as pH decreases from neutral to the acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes.

Use the ready-made pHrodo™ Red E.coli BioParticles™ conjugates in imaging or flow applications, or pHrodo™ Red SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.

Consult user Manual for solubility instructions.

Specifications

Quantity 1 mg
Product Type Succinimidyl Ester
Molecular Weight (g/mol) ∼650
Content And Storage Store in freezer (-5°C to -30°C) and protect from light.
Chemical Reactivity Amine
Shipping Condition Room Temperature
Reactive Moiety Active Ester, Succinimidyl Ester
Label or Dye pHrodo™ Red
Label Type Classic Dyes
Color Red
Product Line pHrodo
Emission 587 nm
Excitation 560 nm
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For Research Use Only. Not for use in diagnostic procedures.