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Invitrogen™ Human NFkB p65 (Phospho) [pS536] InstantOne™ ELISA Kit

Catalog No. 50246258
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96 Tests
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50246258 96 Tests
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Catalog No. 50246258 Supplier Invitrogen™ Supplier No. 858608211

Description

ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human NFkB p65 in cell lysates. Detection of mouse and rat NFkB is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.

Specifications

Accession Number Q04206
Conjugate HRP
Product Type ELISA
Sample Type Cell Lysates
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 5970
Gene Symbol p65,NFKB3
Kit Contents Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 20 μL
Storage Requirements 2°C to 8°C
Synonym RELA, NFKB3, NFkappaB, p65
Target NFkB p65
Test Time 1 hrs 20 mins
Total Assay Time 1 hrs 10 mins
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