Invitrogen™ Phospho-ERK1/ERK2 (Thr202, Tyr204, Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (SC58-01)

Description

Positive Control: SH-SY5Y cell lysate, SH-SY5Y treated with 100 ng/mL hBeta-NGF for 10 minutes cell lysate, PC-12 treated with 100 ng/mL hBeta-NGF for 10 minutes cell lysate, SK-Br-3 whole cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 200 nM PMA for 30 minutes cell lysate, HeLa treated with 200 nM PMA for 30 minutes cell lysate, human thyroid carcinoma tissue. Tissue Specificity: Low tissue specificity. Subcellular Location: Cytoplasm, Nucleus. Sequence Similarities: 100% Mouse/Rat. Predicted band size: 41/43 kDa.

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.