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  6. Invitrogen™ Phospho-BTK/ITK (Tyr551, Tyr511) Recombinant Mouse Monoclonal Antibody (M4G3LN), Alexa Fluor™ Plus 488

Invitrogen™ Phospho-BTK/ITK (Tyr551, Tyr511) Recombinant Mouse Monoclonal Antibody (M4G3LN), Alexa Fluor™ Plus 488

Catalog No. 740047MP488
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50 μL
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740047MP488 50 μL
5000102333 20 μL
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Catalog No. 740047MP488 Supplier Invitrogen™ Supplier No. 740047MP488

Description

Mouse Recombinant Monoclonal Antibody

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser.

In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.

Specifications

Antigen Phospho-BTK/ITK (Tyr551, Tyr511)
Applications Flow Cytometry
Classification Recombinant Monoclonal
Clone M4G3LN
Concentration 1 mg/mL
Conjugate Alexa Fluor Plus 488
Formulation Proprietary buffer with , and 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.3-7.3
Gene ITK
Gene Accession No. Q06187, Q08881
Gene Alias agammaglobulinaemia tyrosine kinase; agammaglobulinemia tyrosine kinase; AGMX1; AI528679; AT; ATK; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; bruton tyrosine kinase; Bruton's tyrosine kinase; BTK; dominant-negative kinase-deficient Brutons tyrosine kinase; Emt; homolog of mouse T-cell itk/tsk; IL2 inducible T cell kinase; IL2 inducible T-cell kinase; IL-2-inducible T cell kinase; IL-2-inducible T-cell kinase; IL2-inducible T-cell kinase; IMD1; interleukin-2-inducible T cell kinase; interleukin-2-inducible T-cell kinase; ITK; Kinase EMB; kinase EMT; Kinase TLK; LPFS1; LYK; PSCTK1; PSCTK2; T-cell-specific kinase; Tcsk; Tlk; truncated Bruton agammaglobulinemia tyrosine kinase; Tsk; Tyrosine-protein kinase BTK; Tyrosine-protein kinase BTK (Brutons tyrosine kinase) (Agammaglobulinaemia tyrosine kinase) (ATK) (B cell progenitor kinase) (BPK) (Kinase EMB); tyrosine-protein kinase BTK isoform (lacking exon 13 to 17); tyrosine-protein kinase BTK isoform (lacking exon 14); tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk; xid; XLA; X-linked immune deficiency
Gene Symbols BTK, ITK
Host Species Mouse
Purification Method Protein A/G
Quantity 50 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 3702, 695
Target Species Human
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2b κ
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