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Invitrogen™ Phospho-beta Tubulin (Ser172) Polyclonal Antibody
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Catalog No. PIPA5143784
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PIPA5143784 100 μL
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Catalog No. PIPA5143784 Supplier Invitrogen™ Supplier No. PA5143784

Description

Rabbit Polyclonal Antibody

This antibody was cross-adsorbed to unphosphorylated betaIII-Tubulin (Ser-172) peptide before affinity purification using phospho-betaIII-Tubulin (Ser-172) peptide (without carrier). The antibody detects a 50 kDa* protein corresponding to the molecular mass of beta-Tubulin on SDS-PAGE immunoblots of purified brain tubulin phosphorylated with ERK2 kinase.

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.

Specifications

Antigen Phospho-beta Tubulin (Ser172)
Applications Western Blot, Immunocytochemistry
Classification Polyclonal
Concentration 0.25 mg/mL
Conjugate Unconjugated
Formulation PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene Tubb4a
Gene Accession No. A2AQ07, P04350, P07437, P69897, P85108, P99024, Q13509, Q13885, Q3KRE8, Q3ZCM7, Q4QRB4, Q7TMM9, Q922F4, Q9BUF5, Q9BVA1, Q9CWF2, Q9D6F9, Q9ERD7, Q9H4B7
Gene Alias 2310057H16Rik; 2810484G07Rik; 3200002H15Rik; AA408537; AI325297; AI596182; B130022C14Rik; bA631M21.2; BB220206; beta 5-tubulin; beta Ib tubulin; beta tubulin; beta tubulin 1; beta-4; beta-4 tubulin; beta-4-tubulin; beta-5; beta-5 tubulin; beta5-tubulin; betaIII-tubulin; beta-tubulin; beta-tubulin class-III; Beta-tubulin class-V; beta-tubulin class-VI; Beta-tubulin isotype I; c(beta)7 tubulin; CDCBM; CDCBM1; CDCBM5; CDCBM6; CFEOM3; CFEOM3A; class I beta tubulin; Class I beta-tubulin; class IIa beta-tubulin; class III beta-tubulin; class IVa beta-tubulin; class V beta tubulin; class V beta-tubulin; CSCSC1; dJ40E16.7; dystonia 4, torsion (autosomal dominant); DYT4; FEOM3; HSA10p15 beta-tubulin 4Q; HsT1601; hypothetical protein LOC540236; hypothetical protein LOC550458; I79_000194; I79_012097; LOC100349792; LOC100761889; LOW QUALITY PROTEIN: tubulin beta-6 chain; M(beta)1; M(beta)2; M(beta)3; M(beta)4; M(beta)5; M(beta)6; M40; M40 antibody; MGC128415 protein; MGC137802 protein; neuron-specific class III beta-tubulin; OK/SW-cl.56; OOMD; OOMD2; RGD1305887; si:ch211-122i22.4; si:ch211-9a18.2; Tubb; tubb beta-5; tubb.L; Tubb1; TUBB2; Tubb2a; tubb2a {ECO:0000250; Tubb3; TUBB4; TUBB4A; tubb5; TUBB-5; TUBB6; TUBB8; Tubulin 5 beta; tubulin beta; tubulin beta 1 class VI; tubulin beta 2A class IIa; tubulin beta 3 class III; tubulin beta 4'; tubulin beta 4A class IVa; tubulin beta 6 class V; tubulin beta 8 class VIII; Tubulin beta chain; tubulin beta class I; tubulin beta class I L homeolog; Tubulin beta class IIa; Tubulin beta class V; tubulin beta MGC4083; tubulin beta-1; tubulin beta-1 chain; tubulin beta-1 chain-like; tubulin beta-2A chain; tubulin beta-2B chain; tubulin beta-3 chain; Tubulin beta-4 chain; tubulin beta-4A chain; Tubulin beta-5 chain; tubulin beta-5 chain; tubulin beta-6 chain; tubulin beta-6 chain; Tubulin beta-7 chain; tubulin beta-8 chain; Tubulin beta-III; tubulin, beta 1 class VI; tubulin, beta 2; tubulin, beta 2A; tubulin, beta 2A class IIA; tubulin, beta 3; tubulin, beta 3 class III; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta 4A class IVa; tubulin, beta 5; tubulin, beta 5 class I; tubulin, beta 6 class V; tubulin, beta class I; tubulin, beta family 1; tubulin, beta polypeptide; tubulin, beta polypeptide 2; tubulin, beta, 3; tubulin, beta, 4; tubulin, beta, 5; uncharacterized protein LOC550458; UniProtKB:Q7TMM9}; Unknown (protein for MGC:134115); unnamed protein product; XELAEV_18039222mg; XLOT; zbetaI; zgc:112515
Gene Symbols TUBB, Tubb1, Tubb2a, TUBB2B, Tubb3, Tubb4a, Tubb5, Tubb6, TUBB8
Host Species Rabbit
Immunogen Phospho-betaIII-Tubulin (Ser-172) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 172 of human betaIII-Tubulin. This sequence is identical to similar regions in betaI, betaII, and betaIII-Tubulin isotypes, and is well conserved in tubulins from most eukaryotic species.
Purification Method Antigen affinity chromatography
Quantity 100 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 10381, 10382, 203068, 22151, 22152, 22153, 22154, 246118, 291081, 29213, 29214, 307351, 347688, 347733, 498736, 545486, 679312, 67951, 7280, 73710, 81027, 84617
Target Species Human, Mouse, Rat
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Product Type Antibody
Form Liquid
Isotype IgG
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