Invitrogen™ eBioscience™ Phalloidin eFluor™ 520

Staining with Phalloidin eFluor™ 520 may be performed at the same time as fluorophore-conjugated antibodies. For best results visualize phalloidin-stained cells within 1 hour of staining.

Phalloidin is a peptide toxin isolated from the Amanita phalloides mushroom. Phalloidin selectively binds F-actin and functions to stablilize actin polymers by inhibiting the dissociation of actin monomers from the filament ends. Because F-actin polymers compose the cytoskeleton of almost all eukaryotic cells, phalloidin conjugates can be useful probes for visualizing the structure of live or fixed cells using microscopy. Phalloidin eFluor 520 can be used to label cells of multiple species. Its exitation and emission is similar to Alexa Fluor 488 and FITC.

Phalloidin eFluor 520 is supplied as a frozen liquid. To prepare the 1000X stock solution, thaw the vial, add 30μL high-quality anhydrous DMSO, mix and briefly spin down. The resulting solution (phalloidin DMSO stock) should be aliquoted, protected from light and moisture, and stored at -20°C. To prepare a 1X working solution of phalloidin conjugate, add 1μL of 1000X phalloidin DMSO stock solution to 1mL of PBS containing 1% BSA.