missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Immunoassay Reagents and Kits
  4. Life Science Research ELISA Kits
  5. Invitrogen™ Human PGI ELISA Kit

Invitrogen™ Human PGI ELISA Kit

Catalog No. EHPGI
Encompass
Change view
Click to view available options
Quantity:
96 Tests
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
EHPGI 96 Tests
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. EHPGI Supplier Invitrogen™ Supplier No. EHPGI

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

ELISA

The Human Pepsinogen I (Hu PGI) ELISA quantitates Hu PGI in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PGI. Principle of the method The Human PGI solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Pepsinogen is the inactive precursor form of Pepsin, one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsinogen is synthesized in the gastric mucosa by chief cells. After secretion, pepsinogen is activated in the presence of HCl, which creates an acidic environment in the stomach, allowing pepsinogen to unfold and cleave itself in an autocatalytic fashion. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus.

Specifications

Accession Number P0DJD7
Assay Range 20-6000 pg/mL
Assay Sensitivity 20 pg/mL
Calibration Range 20 pg/mL to 6,000 pg/mL
Content And Storage Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Assay Solid-phase sandwich ELISA
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Protein Family Other Proteins
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA pair antibody detects human PG I. Other species not determined.
Substrate Type Adaptor Proteins
Sufficient For 96 assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias Pepsin A-4; pepsinogen 4, group I (pepsinogen A); pepsinogen A4; pepsinogen-4; PGA4Pepsin A-4; pepsinogen 4, group I (pepsinogen A); pepsinogen A4; pepsinogen-4; PGA4; PEPC; PGII
Gene ID (Entrez) 643847
Gene Symbol PGA4
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Protein Aliases Pepsinogen C
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Other Research
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Synonym N/A
Target PGI
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
Show More Show Less