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OPTIZYME™ NsiI (Mph1103I) Fisher BioReagents™
Supplier: Thermo Fisher Scientific BP80581
Description
5'...A T G C A^T...3'
3'...T^A C G T A...5'
Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 0 – 20%
- Buffer 2: 50 - 100%
- Buffer 3: 20 - 50%
- Buffer 4: 50 - 100%
- Buffer 5: 100%
- 10mM Tris-HCl (pH 7.4 at 25°C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
Specifications
| 10 U/μL | |
| 7.4 | |
| 10mM Tris HCl (pH 7.4 at 25°C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol | |
| 1000 U | |
| NsiI |
| 25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride | |
| >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I | |
| Keep container tightly closed | |
| ATGCA.T | |
| Liquid |