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OPTIZYME™ MspI (HpaII), Fisher BioReagents™
Supplier: Thermo Fisher Scientific BP80481
Description
5'...C^C G G...3'
3'...G G C^C...5'
Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 50 - 100%
- Buffer 2: 50 - 100%
- Buffer 3: 0 - 20%
- Buffer 4: 100%
- Buffer 5: 0 - 20%
- 10mM potassium phosphate (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
- When the external C in the sequence: CCGG is methylated, both MspI and HpaII cannot cleave. MspI is an isoschizomer of HpaII. However, unlike HpaII, MspI can cleave the sequence when the internal C residue is methylated.
- CCGG
Specifications
| 10 U/μL | |
| 37°C | |
| Gene cloning and DNA genotyping | |
| 3000 U | |
| MspI |
| 10X OPTIZYME™ Buffer 4 | |
| 7.4 | |
| 10mM Potassium Phosphate (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol | |
| C.CGG | |
| Liquid |