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OPTIZYME™ MspI (HpaII), Fisher BioReagents™
Supplier: Thermo Fisher Scientific BP80481
Alert:
This chemical may require us to obtain additional information for our regulatory and chemical compliance records. If required, we will contact you for this information once your order is placed.
Description
5'...C^C G G...3'
3'...G G C^C...5'
Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 50 - 100%
- Buffer 2: 50 - 100%
- Buffer 3: 0 - 20%
- Buffer 4: 100%
- Buffer 5: 0 - 20%
- 10mM potassium phosphate (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
- When the external C in the sequence: CCGG is methylated, both MspI and HpaII cannot cleave. MspI is an isoschizomer of HpaII. However, unlike HpaII, MspI can cleave the sequence when the internal C residue is methylated.
- CCGG
Greener Choice Claims
- Environmental benefits include:
- Renewable Energy
Specifications
| 10 U/μL | |
| 37°C | |
| Gene cloning and DNA genotyping | |
| 3000 U | |
| MspI |
| 10X OPTIZYME™ Buffer 4 | |
| 7.4 | |
| 10mM Potassium Phosphate (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol | |
| C.CGG | |
| Liquid |