Invitrogen™ NTF4 Polyclonal Antibody, Biotin, PeproTech®

Description

AA Sequence of recombinant protein: GVSETAPASR RGELAVCDAV SGWVTDRRTA VDLRGREVEV LGEVPAAGGS PLRQYFFETR CKADNAEEGG PGAGGGGCRG VDRRHWVSEC KAKQSYVRAL TADAQGRVGW RWIRIDTACV CTLLSRTGRA. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human NT-4. Anti-Human NT-4-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hNT-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human NT-4 (500-P83G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hNT-4. Western Blot: To detect hNT-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hNT-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P83GBT-1MG will be provided as 2 x 500 μg.

This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood.