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Invitrogen™ Novex™ TNF-α Human ELISA Kit DFS Item

Each ready-to-use kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency

$420.80 - $1,462.65

Specifications

Gene ID (Entrez) 7124
Sample Size 100μL
Sample Type Plasma, Serum, Supernatants
Sensitivity <1.7pg/mL
Target tumor necrosis factor
View More Specs

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes: Human TNF-α Antibody Coated 96-Well Plate
Human TNF-α Standard
Standard Diluent Buffer
Human TNF-α Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer Concentrate (25X)
Stabilized Chromogen, TMB
Stop Solution
Plate Covers
etailed protocol with validation tests


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Specifications

Description

Research use only kit designed to detect and quantify the level of human TNF-α in serum, plasma, and supernatant using 96-well plates and a microplate reader.

Easy-to-Run Sandwich ELISA
A monoclonal capture antibody specific for Human TNF-α has been machine coated onto the wells of the 96-well plate, ensuring low well-to-well variability and eliminating the need for overnight coating. Samples, including a standard of known Human TNF-α content, controls, and unknowns, are pipetted into these wells. During the first incubation, Human TNF-α antigen binds to the immobilized capture antibody on one site. After washing, a biotinylated monoclonal antibody specific for Human TNF-α is added. During the second incubation, this antibody binds to the immobilized Human TNF-α antigen at a different site. After washing, a streptavidin-horseradish peroxidase (HRP) is added. This binds to the biotinylated detection antibody to complete the four–member sandwich. After a second incubation and washing to remove all unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Human TNF-α present in the original specimen and the optical density can be read on a standard microplate reader. Total assay incubation time is only four hours.

  • Easy to run
  • Rapid protocol
  • Convenient precoated, 96-well plate supplied as removable 8-well strips to accommodate smaller samples run

See protocol insert for more information on validation.

Apoptosis, Apoptosis Related Factors, Cell Analysis, Cell Signaling, Cell Viability, Proliferation & Function, Immunoassays, Ready-To-Use Immunoassay

Order Info

Shipping Condition: Dry Ice

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Description & Specifications

Specifications

7124
Plasma, Serum, Supernatants
tumor necrosis factor
100μL
<1.7pg/mL