Invitrogen™ Novex™ TNF-α Human ELISA Kit
Each ready-to-use kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency
$526.00 - $2,090.00
Research use only kit designed to detect and quantify the level of human TNF-α in serum, plasma, and supernatant using 96-well plates and a microplate reader.
Easy-to-Run Sandwich ELISA
A monoclonal capture antibody specific for Human TNF-α has been machine coated onto the wells of the 96-well plate, ensuring low well-to-well variability and eliminating the need for overnight coating. Samples, including a standard of known Human TNF-α content, controls, and unknowns, are pipetted into these wells. During the first incubation, Human TNF-α antigen binds to the immobilized capture antibody on one site. After washing, a biotinylated monoclonal antibody specific for Human TNF-α is added. During the second incubation, this antibody binds to the immobilized Human TNF-α antigen at a different site. After washing, a streptavidin-horseradish peroxidase (HRP) is added. This binds to the biotinylated detection antibody to complete the four–member sandwich. After a second incubation and washing to remove all unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Human TNF-α present in the original specimen and the optical density can be read on a standard microplate reader. Total assay incubation time is only four hours.
- Easy to run
- Rapid protocol
- Convenient precoated, 96-well plate supplied as removable 8-well strips to accommodate smaller samples run
See protocol insert for more information on validation.
Apoptosis, Apoptosis Related Factors, Cell Analysis, Cell Signaling, Cell Viability, Proliferation & Function, Immunoassays, Ready-To-Use Immunoassay
Shipping Condition: Dry Ice
This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.
|Plasma, Serum, Supernatants|
|tumor necrosis factor|