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Invitrogen™ Streptavidin Agarose

Catalog No. SA10004
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SA10004 5 mL
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Catalog No. SA10004 Supplier Invitrogen™ Supplier No. SA10004
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Description

Includes

Streptavidin Agarose is supplied as 10mL of a 50% slurry containing 5mL of packed gel bed in 0.01M sodium phosphate (pH 7.2), 0.15M NaCl, and 0.02% sodium azide.

For isolating biotinylated molecules and complexes with biotinylated components, including antibodies, antibody-antigen complexes, ligands and receptors, nucleic acids, and nucleic acid-protein complexes

  • Especially useful as an immunoprecipitation reagent for antibodies that do not bind well to protein A
  • The matrix consists of streptavidin covalently attached to cross-linked 4% agarose beads through a 15-atom spacer arm
  • Streptavidin Agarose can bind 3 to 8mg of biotinylated IgG/mL of gel

Immunoprecipitation, Protein and Peptide Purification, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis

Specifications

Concentration 10 mg/mL
Quantity 5 mL
Content And Storage Streptavidin Agarose is supplied as 10 ml of a 50% slurry containing 5 ml of packed gel bed in 0.01 M sodium phosphate (pH 7.2), 0.15 M NaCl, and 0.02% sodium azide. Store at 4°C.
Form Liquid Suspension
Product Type Streptavidin Agarose

Frequently Asked Questions (FAQs)

How many biotin binding-sites are there per streptavidin molecule?

Streptavidin is a protein composed of four identical subunits, each containing a high affinity binding site for biotin (K-D = 10 -15 M) . Streptavidin has the same biotin binding properties as avidin, but it has a low isoelectric point (pI=5) and no carbohydrate groups, resulting in low non-specific binding.
What is the binding capacity of streptavidin agarose?

The binding capacity of streptavidin agarose is 15-20 µg (18-24 nmoles) of fluorescein biotin per mL of sedimented gel.

For Research Use Only. Not for use in diagnostic procedures.