Please login to your online account to display your discounted pricing

Invitrogen™ Novex™ phosphoELISA™ TAU (Total) Human ELISA Kit DFS Item

Designed to detect and quantify the level of human Tau protein, regardless of phosphorylation state

$619.50 - $1,073.10

Specifications

Concentration 31-2000pg/mL
Conjugate HRP (Horseradish Peroxidase)
Gene ID (Entrez) 4137
Marker Gene Tau
Sample Type Cerebrospinal Fluid, Supernatants
View More Specs
Includes:  Pre-coated plate, standard, biotinylated detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

KHB0042

DFS Item
 
invitrogen™
KHB0042
192 assays Each for $1,073.10

KHB0041

DFS Item
 
invitrogen™
KHB0041
96 assays Each for $619.50
Description & Specifications

Specifications

Concentration 31-2000pg/mL
Conjugate HRP (Horseradish Peroxidase)
Gene ID (Entrez) 4137
Marker Gene Tau
Sample Type Cerebrospinal Fluid, Supernatants
Sample Volume 50μL
Sensitivity <12pg/mL
Target Microtubule-associated protein tau

The Tau (Total) Human ELISA Kit is intended for detection of Tau from cerebral spinal fluid (CSF) or from tissue culture supernatant using 96-well plates and a microplate reader.

Easy-to-run sandwich ELISA
A monoclonal capture antibody specific for Hu Tau has been coated onto the wells of the 96-well plate provided. During the first incubation, standards of known Tau content and unknown samples are pipetted into the wells, and the Tau antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for Tau is added to the wells. During the second incubation, this antibody serves as a detection antibody by binding to the immobilized Tau protein captured during the first incubation. After washing, a horseradish peroxidase-labeled, anti-rabbit IgG is added. This binds to the detection antibody to complete the four-member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Hu Tau present in the original specimen and the optical density can be read on a standard microplate reader.

Convenient kit format
The kit comes with a capture antibody precoated onto the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips, allowing you to run as few samples as you want. Standards provided in the kit can be used to obtain quantitative results from a standard curve or as a positive control.

  • Easy to run
  • Convenient precoated, removable 8-well strips
  • Validated kits for accuracy and consistency
  • Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency

See protocol insert for more information on validation.

Cell Analysis, Immunoassays, Ready-To-Use Immunoassay

Shipping condition: Wet ice

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.