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Invitrogen™ Cell Lysis Buffer II DFS Item

Cell Lysis Buffer II is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications.

Manufacturer:  Invitrogen™ FNN0021

Catalog No. FNN0021



Description

Description

  • Buffer is used for total protein extraction and utilizes detergent-based lysis, eliminating the need for mechanical cell disruption
  • Formulation helps retain the protein structure and function needed for enzyme assays or immunoassays
  • Buffer formulation: 50 mM Tris, pH 7.4, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 mM Na3VO4, 1% detergent, 0.02% NaN3
Note: does not contain protease inhibitors and should be supplemented with 1 mM PMSF and protease inhibitor cocktail just prior to use.

Order Info

Shipping condition: Dry ice

Specifications

Specifications

Store in freezer (-5 to -30°C).
Cell Lysis Buffer
100 mL
Dry Ice
SDS
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For Research Use Only. Not for use in diagnostic procedures.