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Invitrogen™ Ni-NTA Purification System

Catalog No. K95001
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6 purifications
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Catalog No. Quantity
K95001 6 purifications
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Catalog No. K95001 Supplier Invitrogen™ Supplier No. K95001
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Description

Includes

6 x 2mL resin columns and buffers for native and denaturing purification; 10mL Ni-NTA Agarose.

Designed for the purification of recombinant proteins that contain a polyhistidine (6xHis) sequence

  • Utilizes Ni-NTA nickel-chelating resin and is supplied with native and denaturing buffers for efficient purification of recombinant proteins under different conditions

His-Tagged Protein Purification, Protein and Peptide Purification, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis

Specifications

Format Suspension
Product Type Ni-NTA Purification System
Content And Storage Six 2-ml resin columns and buffers for native and denaturing purification. Ten milliliters of Ni-NTA Agarose. Store at +4°C. All reagents are guaranteed stable for 6 months when properly stored.
Protein Tag His Tag
Quantity 6 purifications

Frequently Asked Questions (FAQs)

Can ProBond or Ni-NTA beads be used for large-scale preparations?

ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).
What should the typical protein recovery be when using the Probond Purification System or Ni-NTA Purification system?

Both systems are qualified by purifying 2 mg of myoglobin protein on a column and performing a Bradford assay. Protein recovery must be 75% or higher.

For Research Use Only. Not for use in diagnostic procedures.