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Invitrogen™ MMP2 Human ELISA Kit 
Detects and quantifies protein levels of human MMP-2 in serum, plasma, and cell culture supernatant
$692.00 - $1218.00
Specifications
| Accession Number | P08253 |
|---|---|
| Assay Range | 0.78-50 ng/mL |
| Assay Sensitivity | <1 ng/mL |
| Conjugate | Biotin |
| Product Type | ELISA Kit |
Includes: Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers
Description
The Human Matrix MetalloProteinase 2 (Hu MMP2) ELISA quantitates Hu MMP2 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MMP2. Principle of the method The Human MMP2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Matrix metalloproteinase-2 (MMP-2), together with MMP-9, degrades type IV collagen.
Cell Analysis, Immunoassays, Ready-To-Use Immunoassay
Order Info
Shipping Condition: Dry Ice
Specifications
| P08253 | |
| <1 ng/mL | |
| ELISA Kit | |
| Human | |
| Colorimetric Microplate Reader | |
| CLG4,CLG4A,MMP-2,MMP-II,MONA,TBE-1 | |
| 5.6% | |
| Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| MMP-2 | |
| Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL | |
| Human | |
| 4 hr. |
| 0.78-50 ng/mL | |
| Biotin | |
| Plasma, Serum, Supernatant | |
| Colorimetric Detection | |
| 4313 | |
| 4 hr. | |
| 3.3% | |
| HRP | |
| RUO | |
| 2°C to 8°C | |
| 1 hr. 20 min. |
For Research Use Only. Not for use in diagnostic procedures.