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Invitrogen™ IL-4 Human Matched Antibody Pair

Numéro de catalogue. CHC1283
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10 x 96 Tests
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Numéro de catalogue.
CHC1283 10 x 96 Tests
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Numéro de catalogue. CHC1283 Fournisseur Invitrogen™ Code fournisseur CHC1283

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Comprend

Antibody Pair Kit contains matched, pre-titred and fully optimized Capture (coating) and Detection Antibodies, 3 vials of Recombinant Standard, Streptavidin-HRP concentrate and technical data sheet.

Exige

Buffer reagents required to complete reaction available separately (Cat. No. CNB0011).

Matched Antibody Pair

The Human IL-4 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-4. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

Order Info

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Spécifications

Conjugué HRP
Numéro d’acquisition du gène P05112
Alias du gène BCGF-1, BCGF1, BSF-1, BSF1, IL-4, MGC79402
Symboles de gène IL4
Quantité 10 x 96 Tests
ID du gène (Entrez) 3565
Comprend Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Espèces cibles Human
Contenu et stockage 2°C to 8°C
Type de produit ELISA Kit
À utiliser avec (équipement) Colorimetric Microplate Reader
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For Research Use Only. Not for use in diagnostic procedures.