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  5. Invitrogen™ Human IL-8 ELISA Kit, Ultrasensitive

Invitrogen™ Human IL-8 ELISA Kit, Ultrasensitive

Numéro de catalogue. KHC0084
Encompass
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Quantité:
2 x 96 Tests
96 Tests
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Numéro de catalogue. Quantité
KHC0084 96 Tests
KHC0083 2 x 96 Tests
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Numéro de catalogue. KHC0084 Fournisseur Invitrogen™ Code fournisseur KHC0084

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Description

Comprend

Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

ELISA

The Human Interleukin 8 (IL-8) ELISA quantitates Hu IL-8 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-8. Principle of the method The Human IL-8solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.

Order Info

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Spécifications

Numéro d'enregistrement P10145
Plage de l'essai 0.39 to 25 pg/mL
Sensibilité d’essai <100 fg/mL
Conjugué HRP
Type de produit ELISA
Type d’échantillon Plasma, Serum, Supernatant
Espèces cibles Human
À utiliser avec (application) Colorimetric
À utiliser avec (équipement) Colorimetric Microplate Reader
ID du gène (Entrez) 3576
Symbole de gène GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1
CV intra-essai 7.6%, 5.8
Contenus des kits Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Étiquette ou colorant HRP
Precision <0.1 pg/mL
Quantité 96 Tests
Statut réglementaire RUO
Volume d’échantillon Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Conditions de stockage 2°C to 8°C
Cible IL-8
Durée du test 1 hrs 20 mins
Durée totale de l'essai 4 hrs
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For Research Use Only. Not for use in diagnostic procedures.