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Invitrogen™ Novex IL-8 Human ELISA Kit DFS Item

Designed to detect and quantify the level of human IL-8 in serum, plasma, and supernatant using 96-well plates and a microplate reader

$526.00 - $2,090.00

Specifications

For Use With (Application) Colorimetric
Gene ID (Entrez) 3576
Precision <5pg/mL
Regulatory Status RUO - research use only
Sample Size 50μL
View More Specs

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes: Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.


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Specifications

Description

  • The total assay incubation time is only 2.5 hours
  • A monoclonal capture antibody specific for Human IL-8 has been coated onto the wells of the 96-well plate provided
  • Samples, including a standard of known Human IL-8 content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinylated polyclonal detector antibody
  • During the first incubation, Human IL-8 antigen binds simultaneously to the immobilized capture antibody and to the solution phase biotinylated detector antibody on a second site
  • After washing, a streptavidin-horseradish peroxidase (HRP) is added
  • This binds to the biotinylated detection antibody to complete the four–member sandwich
  • After a second incubation and washing to remove all unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color
  • The intensity of this colored product is directly proportional to the concentration of Human IL-8 present in the original specimen and the optical density can be read on a standard microplate reader

Convenient Kit Format
The kit comes with a capture antibody precoated to the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips allowing you to run as few samples as you want. Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency.

Cell Analysis, Immunoassays, Ready-To-Use Immunoassay

Order Info

Shipping Condition: Wet ice

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Description & Specifications

Specifications

Colorimetric
<5pg/mL
50μL
Interleukin 8
3576
RUO - research use only
Plasma, Serum, Supernatants
2.5 hrs