missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

Invitrogen™ Novex™ IFN-γ Human ELISA Kit DFS Item

Each ready-to-use kit ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency.

$526.00 - $2,090.00


Gene ID (Entrez) 3458
Sample Size 50μL
Sample Type Serum, Supernatants
Sensitivity <4pg/mL
Target interferon, gamma
View More Specs


For Research Use Only. Not for use in diagnostic procedures.

Includes: Human IFN-γ Antibody Coated 96-Well Plate
Human IFN-γ Standard
Standard Diluent Buffer
Human IFN-γ Detection Antibody
HRP Diluent
Wash Buffer Concentrate (25X)
Stabilized Chromogen, TMB
Stop Solution
Plate Covers
Detailed protocol with validation tests
Plate precoated with capture antibody

Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
KHC4021 Invitrogen™
96 assays Each for $526.00
KHC4021C Invitrogen™
480 assays Each for $2,090.00
KHC4022 Invitrogen™
192 assays Each for $980.00


Detects and quantifies the level of human IFN-γ in serum and supernatant using 96-well plates and a microplate reader. Convenient precoated, 96-well plate supplied as removable 8-well strips to accommodate smaller samples run. Easy-to-run sandwich ELISA has a total assay incubation time is only 2.75 hours. A monoclonal capture antibody specific for Human IFN-γ machine coated onto the wells of the 96-well plate, ensuring low well-to-well variability and eliminating the need for overnight coating. Samples, including a standard of known Human IFN-γ content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinylated polyclonal detector antibody. During the first incubation, Human IFN-γ antigen binds simultaneously to the immobilized capture antibody and to the solution phase biotinylated detector antibody on a second site. After washing, a streptavidin-horseradish peroxidase (HRP) is added. This binds to the biotinylated detection antibody to complete the four–member sandwich. After a second incubation and washing to remove all unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Human IFN-γ present in the original specimen and the optical density can be read on a standard microplate reader.

See protocol insert for more information on validation.

Cell Analysis, Cell Signaling, Cell Viability, Proliferation & Function, Immunoassays, Ready-To-Use Immunoassay

Order Info

Shipping Condition: Room Temperature

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Description & Specifications


Serum, Supernatants
interferon, gamma