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Invitrogen™ Novex™ IFN-γ Human ELISA Kit DFS Item

Each ready-to-use kit ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency.

$525.00 - $2,089.50

Specifications

Gene ID (Entrez) 3458
Sample Size 50μL
Sample Type Serum, Supernatants
Sensitivity <4pg/mL
Target interferon, gamma
View More Specs
Includes:  Human IFN-γ Antibody Coated 96-Well Plate
Human IFN-γ Standard
Standard Diluent Buffer
Human IFN-γ Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer Concentrate (25X)
Stabilized Chromogen, TMB
Stop Solution
Plate Covers
Detailed protocol with validation tests
Plate precoated with capture antibody

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

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KHC4021

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KHC4022

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Description & Specifications

Specifications

Gene ID (Entrez) 3458
Sample Size 50μL
Sample Type Serum, Supernatants
Sensitivity <4pg/mL
Target interferon, gamma

Detects and quantifies the level of human IFN-γ in serum and supernatant using 96-well plates and a microplate reader. Convenient precoated, 96-well plate supplied as removable 8-well strips to accommodate smaller samples run. Easy-to-run sandwich ELISA has a total assay incubation time is only 2.75 hours. A monoclonal capture antibody specific for Human IFN-γ machine coated onto the wells of the 96-well plate, ensuring low well-to-well variability and eliminating the need for overnight coating. Samples, including a standard of known Human IFN-γ content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinylated polyclonal detector antibody. During the first incubation, Human IFN-γ antigen binds simultaneously to the immobilized capture antibody and to the solution phase biotinylated detector antibody on a second site. After washing, a streptavidin-horseradish peroxidase (HRP) is added. This binds to the biotinylated detection antibody to complete the four–member sandwich. After a second incubation and washing to remove all unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Human IFN-γ present in the original specimen and the optical density can be read on a standard microplate reader.

See protocol insert for more information on validation.

Cell Analysis, Cell Signaling, Cell Viability, Proliferation & Function, Immunoassays, Ready-To-Use Immunoassay

Shipping Condition: Room Temperature

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