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Invitrogen™ Novex™ iBlot™ Nitrocellulose Transfer Stack DFS Item


iBlot Transfer Stacks are used to transfer proteins

Manufacturer: invitrogen™  IB301031

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Catalog No. IB301031

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Description & Specifications

Specifications

Product Type Regular, 13.5 x 8cm; 3 x 10 sets
Material Nitrocellulose, 0.2μm pore size

The iBlotTransfer Stacks are disposable stacks that come in three varieties for different applications: iBlotGel Transfer Stacks have integrated PVDF or nitrocellulose transfer membranes to perform dry blotting of proteins; iBlot DNA Transfer Stacks have an integrated nylon transfer membrane to perform dry blotting of DNA, and iBlot Western Detection Stacks are used to perform Western detection on previously blotted nitrocellulose or PVDF membranes. Each iBlotTransfer Stack contains a copper electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins or DNA.

Nitrocellulose membrane

  • Composed of 100% pure nitrocellulose to provide high-quality transfer
  • Compatible with commonly used detection methods such as staining, immunodetection, fluorescence, or radiolabeling
  • Bind proteins due to hydrophobic and electrostatic interactions
  • Pore size: 0.2μm
  • Protein binding capacity: 209μg/cm2

 

Transfer stack sizes

  • The regular size (13 x 8.3cm) is used for all Novex midi-gel types (or equivalent) and E-PAGE™ 48 or 96 Gels
  • The mini size (8 x 8cm) is used for all Novex mini-gels (or equivalent)

 

Proteins, Expression, Isolation and Analysis, Western Blotting

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