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Invitrogen™ Novex™ HRP Chromogenic Substrate (TMB)

Catalog No. WP20004
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1 x 250 ml bottle
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WP20004 1 x 250 ml bottle
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Catalog No. WP20004 Supplier Invitrogen™ Supplier No. WP20004
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Description

Ready to use single component reagent used for sensitive detection of a horse radish peroxidase (HRP) on western blot and dot blot membranes

Consisting of a ready-to-use solution of TMB, the Novex™ HRP Chromogenic Substrate forms a blue precipatate up reaction with horse radish peroxidase (HRP).

Order Info

Approved for shipment at Room Temperature or on Wet Ice

Specifications

Quantity 1 x 250 ml bottle
Shipping Condition Approved for shipment at Room Temperature or on Wet Ice
Substrate Properties Chemical Substrate
Substrate Type HRP (Horseradish Peroxidase) Substrate
Color Blue
Content And Storage Single bottle labelled: Novex™ HRP Chromogenic Substrate (TMB). Avoid exposure to light. Store at 4°C.
Detection Method Colorimetric
Form Liquid
Product Line Novex
Substrate TMB

Frequently Asked Questions (FAQs)

What are the Invitrogen AP Chromogenic Substrate (BCIP/NBT) and Invitrogen HRP Chromogenic Substrate (TMB)?

- Invitrogen AP Chromogenic Substrate (BCIP/NBT) and Invitrogen HRP Chromogenic Substrate (TMB) are ready-to-use, single component substrates for sensitive immunodetection of alkaline phosphatase (AP) or horse radish peroxidase (HRP), respectively, on western blots or dot blots. The sensitivity of the AP substrate is in the 100 pg range, while the HRP substrate is in the 1 ng range (see Note below).

- Invitrogen AP Chromogenic Substrate consists of a ready-to-use solution of 5-bromo-4-chloro-3-indolyl-1-phosphate (BCIP) and nitroblue tetrazolium (NBT) which forms a black-purple precipitate upon reaction with alkaline phosphatase. It is available as a standalone product (Cat. Nos. WP20001) and is also a component of the WesternBreeze Chromogenic Detection Kits.

- Invitrogen HRP Chromogenic Substrate consists of a ready-to-use non-toxic solution of 3,3',5,5'-tetramethylbenzidine (TMB) which forms a blue precipitate upon reaction with horse radish peroxidase. It is available as a standalone product (Cat. No. WP20004).

Note: Sensitivity may vary depending upon the antigen and antibody used for detection.

For Research Use Only. Not for use in diagnostic procedures.