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Invitrogen™ Human IL-4 ELISA Kit

Catalog No. KAC1281
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96 Tests
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KAC1281 96 Tests
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Catalog No. KAC1281 Supplier Invitrogen™ Supplier No. KAC1281

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Description

ELISA

The Human Interleukin 4 (Hu IL-4) ELISA quantitates Hu IL-4 in human serum. The assay will exclusively recognize both natural and recombinant Hu IL-4. Principle of the method The Human IL-4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

Specifications

Accession Number P05112
Assay Range 14.5 to 1695 pg/mL
Assay Sensitivity 1.2 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Serum
Target Species Human
For Use With (Application) Colorimetric
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 3565
Gene Symbol BCGF-1,BCGF1,BSF-1,BSF1,IL-4
Interassay CV 5.1%
Intraassay CV 3.8
Kit Contents Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls
Label or Dye HRP
Precision 2 pg/mL
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Serum 100 μL
Storage Requirements 2°C to 8°C
Target IL-4
Test Time 1 hrs 20 mins
Total Assay Time 2 hrs 30 mins
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