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  6. Invitrogen™ Novex™ Zymogram Plus (Gelatin) Protein Gels, 10%, 1.0 mm

Invitrogen™ Novex™ Zymogram Plus (Gelatin) Protein Gels, 10%, 1.0 mm

Catalog No. p-7237815
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Catalog No. No. of Wells
ZY00100BOX 10-well
ZY00102BOX 12-well
ZY00105BOX 15-well
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Catalog No. ZY00100BOX Supplier Invitrogen™ Supplier No. ZY00100BOX
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Description

Novex 10% Zymogram Plus (Gelatin) gels are useful for the detection and characterization of proteases that use gelatin as a substrate. The proteases are run under denaturing conditions and visualized as clear bands against a dark background using a simple renaturing, developing, and staining protocol.

Novex 10% Zymogram Plus (Gelatin) gels are useful for the detection and characterization of proteases that use gelatin as a substrate. The proteases are run under denaturing conditions and visualized as clear bands against a dark background using a simple renaturing, developing, and staining protocol. Zymogram gels are commonly used to detect matrix metalloproteinases. Novex 10% Zymogram Plus gels are highly sensitive, detecting as little as 5 x 10-6 units of collagenase.

Formulation
 Novex Zymogram Plus gels are Tris-glycine gels with 0.1% gelatin incorporated into the gel. They are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, highly purified water, and gelatin as the substrate. All have a 4% stacking gel.

Choose the right gel format for your experiments
Novex 10% Zymogram Plus gels come in a fixed concentration of 10% gelatin and three different well formats, including 10-, 12- and 15-well.

Recommended buffers
 Use Novex Tris-Glycine SDS Sample Buffer (LC2676) and Novex Tris-Glycine SDS Running Buffer (LC2675) with these gels. Novex Zymogram Renaturing Buffer (LC2670) and Novex Zymogram Developing Buffer (LC2671) are also recommended.

Recommended Storage

Store in refrigerator (2-8°C). Do not freeze.

Specifications

For Use With (Equipment) Mini Gel Tank, XCell SureLock™ Mini-Cell
Gel Size Mini
Product Line Novex
Gel Percentage 0.1
Gel Type Zymogram Gel
Mode of Separation Molecular Weight
No. of Wells 10-well
Quantity 10 gels (1 box)
Sample Loading Volume Up to 25 μL
Separation Range 30 to 150 kDa (Tris-Glycine Buffer)
Shipping Condition Wet Ice
Temperature (Metric) Storage Store at 2°C to 8°C. Do not freeze.
Thickness (Metric) 1.0 mm
Width (Metric) 8 cm
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