missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Gel Electrophoresis Equipment and Supplies
  4. Gel Electrophoresis Equipment
  5. Electrophoresis System Accessories and Replacement Parts
  6. Invitrogen™ Novex™ Zymogram Plus (Gelatin) Protein Gels, 10%, 1.0 mm

Invitrogen™ Novex™ Zymogram Plus (Gelatin) Protein Gels, 10%, 1.0 mm

Catalog No. ZY00105BOX
Encompass
Change view
Click to view available options
No. of Wells:
10-well
12-well
15-well
3 product options available for selection
Product selection table with 3 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. No. of Wells
ZY00105BOX 15-well
ZY00100BOX 10-well
ZY00102BOX 12-well
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 3 options available.
3 options
Catalog No. ZY00105BOX Supplier Invitrogen™ Supplier No. ZY00105BOX
Only null left

Description

Novex 10% Zymogram Plus (Gelatin) gels are useful for the detection and characterization of proteases that use gelatin as a substrate. The proteases are run under denaturing conditions and visualized as clear bands against a dark background using a simple renaturing, developing, and staining protocol.

Novex 10% Zymogram Plus (Gelatin) gels are useful for the detection and characterization of proteases that use gelatin as a substrate. The proteases are run under denaturing conditions and visualized as clear bands against a dark background using a simple renaturing, developing, and staining protocol. Zymogram gels are commonly used to detect matrix metalloproteinases. Novex 10% Zymogram Plus gels are highly sensitive, detecting as little as 5 x 10-6 units of collagenase.

Formulation
 Novex Zymogram Plus gels are Tris-glycine gels with 0.1% gelatin incorporated into the gel. They are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, highly purified water, and gelatin as the substrate. All have a 4% stacking gel.

Choose the right gel format for your experiments
Novex 10% Zymogram Plus gels come in a fixed concentration of 10% gelatin and three different well formats, including 10-, 12- and 15-well.

Recommended buffers
 Use Novex Tris-Glycine SDS Sample Buffer (LC2676) and Novex Tris-Glycine SDS Running Buffer (LC2675) with these gels. Novex Zymogram Renaturing Buffer (LC2670) and Novex Zymogram Developing Buffer (LC2671) are also recommended.

Recommended Storage

Store in refrigerator (2-8°C). Do not freeze.

Specifications

For Use With (Equipment) Mini Gel Tank, XCell SureLock™ Mini
Gel Size Mini
Product Line Novex
Gel Percentage 0.1
Gel Type Zymogram Gel
Mode of Separation Molecular Weight
No. of Wells 15-well
Quantity 10 gels (1 box)
Sample Loading Volume Up to 15 μL
Separation Range 30 to 150 kDa (Tris-Glycine Buffer)
Shipping Condition Wet Ice
Temperature (Metric) Storage Store at 2°C to 8°C. Do not freeze.
Thickness (Metric) 1.0 mm
Width (Metric) 8 cm
Show More Show Less

Frequently Asked Questions (FAQs)

I ran a Zymogram gel and my bands are blurry and not clear to see. Why is this?

Some small proteases work better if the sample is heated for a short time before loading so the protease is denatured better and it doesn't digest the casein on its way through the gel. This may also help to improve the band sharpness.

What stains do you recommend for Zymogram gels?

Zymogram gels can be stained using Colloidal Blue stain or SimplyBlue SafeStain. The fixing step is not required. We recommend staining with SimplyBlue SafeStain after renaturing and developing the gel for enzyme activity. If greater contrast is needed for detecting weakly active enzymes, a 0.5% Coomassie R250 stain may be used.

Do protein standards run differently on a Zymogram gel compared to a regular Tris-Glycine gel?

Zymogram gels are essentially Tris-Glycine gels containing the substrate. Protein standards run based solely on the percentage of acrylamide and hence should run the same in both kinds of gels. It is quite possible though that if the standard is pre-stained, the proteins will appear a different color because of the staining (or pre-staining) of the Zymogram gels.

Should I boil my sample before running it on a Zymogram gel?

No, samples should not be heated or reduced so that multiunit proteases migrate as a single unit that can be renatured after electrophoresis. The sample should be one part sample and one part 2X Tris-Glycine SDS Sample buffer. Let the sample stand at room temperature for 10 minutes and then load the sample onto the gel.

What are the substrates for the matrix metalloproteases (MMPs)?

The substrates for the matrix metalloproteases (MMPs) are:

*MMP-1: tissue collagenase: collagen 1, 2, 3, 4, 6, & 10

*MMP-2: gelatinase: gelatins, collagens 4, 5, & 7

*MMP-3: stromleysin-2: casein, fibronectin, laminin, elastin

*MMP-7: matrilysin: casein

*MMP-8: neutrophil collagenase: collagen

*MMP-9: type 4 collagenase: gelatin

*MMP-10: stromelysin-2: casein

*MMP-12: metalloelastase: elastin

What are the applications for Zymogram gels?

Invitrogen Zymogram gels are an excellent tool for detecting and characterizing proteases that can utilize casein or gelatin as a substrate. Proteases are run on a gel containing one of these substrates under SDS conditions. They can be visualized as clear bands against a dark background following a simple renaturing and staining protocol. These gels are commonly used to detect matrix metalloproteases, a family of enzymes linked to the pathogenesis of cancer and some inflammatory diseases.

How should I store the Zymogram buffers?

The Zymogram buffers should be stored at 4 degrees C.

What is the shelf life of Zymogram gels?

The shelf life of Zymogram gels is 8 weeks when stored at 4 degrees C.

How should I store my Zymogram gels?

We recommend storing the Zymogram gels at 4 degrees C.

Do your Zymogram gels contain the gelatin or casein substrate in the stacking portion?

Our Zymogram gels do not contain the substrate (gelatin or casein) in the stacking gel although a small amount might mix in during casting of the gel.

Do your Zymogram gels have a stacking gel?

Yes, our Zymogram gels do contain a 4% stacking gel that is ~8 to 9mm long.

What are the recommended sample loading volumes and protein loading amounts for Zymogram gels?

The recommended sample loading volumes and protein loading amounts for the different well formats can be found at: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/recommended-well-loading-volumes-sample-loads.html.

What is the ratio of acrylamide:bisacrylamide and percentage of crosslinker in your Zymogram gels?

The ratio of acrylamide:bisacrylamide in our Zymogram gels is 37.5:1 and percentage of cross-linker is 2.6%.

Do your Zymogram gels contain SDS?

Our Zymogram gels do not contain SDS. We recommend using the Invitrogen Tris-Glycine SDS Sample buffer and the Invitrogen Tris-Glycine SDS Running buffer with these gels. Invitrogen Zymogram Renaturing buffer and Invitrogen Zymogram Developing buffer are recommended for enzyme assays.

What kind of gelatin is present in the Invitrogen 10% Zymogram Gelatin gels?

The Invitrogen 10% Zymogram Gelatin gels are 10% Tris-Glycine gels with 0.1% Type A gelatin from porcine skin (Sigma Cat. No. G2500), as the substrate.