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MilliporeSigma™ Novagen™ Veggie BL21 (DE3) Singles™ Competent Cells

Catalog No. 712524
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Catalog No. 712524 Supplier MilliporeSigma™ Supplier No. 712524
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Protease-deficient hosts (BL21) for protein expression; Manufactured free of animal-derived media and components

BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

Specifications

Content And Storage ≤-70°C
Quantity 22 Reactions