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MilliporeSigma™ Novagen™ pSTBlue-1 AccepTor™ Vector Giga Cloning Kit
Features NovaBlue GigaSingles™ Competent Cells for increased transformation efficiency. MilliporeSigma™; Novagen™ pSTBlue-1 AccepTor™ Vector Giga Cloning Kit is designed for the cloning of PCR products generated using thermostable DNA polymerases that leave single 3'-dA nucleotide overhangs.
Supplier: MilliporeSigma™ 712284
Description
- Vector enables direct ligation by virtue of single 3'-dU overhangs that anneal with 3'-dA extensions on PCR products, the dU residues are converted to dT residues in vivo
- No restriction enzymes or special primers
- Direct ligation of PCR product with vector
- Compatible with polymerases that leave single 3´-dA overhangs
- Blue/white screening with pSTBlue-1 or pETBlue™-1 vectors
- Simple protocol takes as little as 40 minutes from PCR product to plating transformants
Chemical Identifiers
| pSTBlue-1 Vector Giga Cloning Kit | |
| Store at ≤70°C. |
Specifications
| pSTBlue-1 Vector Giga Cloning Kit | |
| Store at ≤70°C. | |
| 40 Reactions |
For Research Use Only