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MilliporeSigma™ Novagen™ pSTBlue-1 AccepTor™ Vector Giga Cloning Kit

Catalog No. 712283
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712284 40 Reactions
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Catalog No. 712283 Supplier MilliporeSigma™ Supplier No. 712283

Description

Features NovaBlue GigaSingles™ Competent Cells for increased transformation efficiency. MilliporeSigma™; Novagen™ pSTBlue-1 AccepTor™ Vector Giga Cloning Kit is designed for the cloning of PCR products generated using thermostable DNA polymerases that leave single 3'-dA nucleotide overhangs.

  • Vector enables direct ligation by virtue of single 3'-dU overhangs that anneal with 3'-dA extensions on PCR products, the dU residues are converted to dT residues in vivo
  • No restriction enzymes or special primers
  • Direct ligation of PCR product with vector
  • Compatible with polymerases that leave single 3´-dA overhangs
  • Blue/white screening with pSTBlue-1 or pETBlue™-1 vectors
  • Simple protocol takes as little as 40 minutes from PCR product to plating transformants

Specifications

Product Type pSTBlue-1 Vector Giga Cloning Kit
Content And Storage Store at ≤70°C.
Quantity 20 Reactions

For Research Use Only