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MilliporeSigma™ Novagen™ Benzonase™ Nuclease

Catalog No. 707463
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10 kU
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707463 10 kU
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Catalog No. 707463 Supplier MilliporeSigma™ Supplier No. 707463
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Description

Used for removal of nucleic acids from recombinant proteins

  • Genetically engineered endonuclease from Serratia marcescens
  • Degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) while having no proteolytic activity
  • Effective over wide range of conditions and possesses high specific activity
  • Rapidly hydrolyzes nucleic acids to makes enzyme for viscosity reduction to reduce processing time and increase yields of protein
  • Compatible with BugBuster™ and PopCulture™ Protein Extraction Reagents
  • Can be added along with these reagents to eliminate viscosity and remove nucleic acids from E. coli extracts
  • Functional between pH 6 and 10 and from 0 to 42°C and requires 1to 2mM Mg2+ for activation
  • Active in presence of ionic and non-ionic detergents, reducing agents, PMSF (1mM), EDTA (1mM) and urea
  • Activity is inhibited by >150mM monovalent cations, >100mM phosphate, >100mM ammonium sulfate, or >100mM guanidine HCl
  • Packaging: Plastic bottle

Compliance

Compliant with FDA

Specifications

Concentration 25 to 29U/μL
For Use With (Application) Nucleic Acids Removal
Formulation Supplied in 50% glycerol
pH Range 6 to 10
Quantity 10 kU
Storage Requirements -20°C
Regulatory Status RUO
Product Type Nuclease
Purity or Quality Grade >90%