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MilliporeSigma™ Novagen™ BCA Protein Assay Kit

Catalog No. 712853
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Catalog No. 712853 Supplier MilliporeSigma™ Supplier No. 712853
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Determines protein concentration in standard assay or microassay configuration.

EMD Millipore Novagen™ BCA Protein Assay Kit is based on biuret reaction, which is the reduction of Cu2+ to Cu+ by proteins in alkaline solution.

  • Sufficient to complete 500 standard-size reactions or or 2500 microscale reactions
  • Used to determine protein concentrationin the range of 20-2000 μg/mL
  • Robust, can be performed in presence of many compounds
  • BSA standard (2 mg/mL) is provided for convenient and reliable preparation of standard curves

Specifications

Sufficient For 500 standard-size reactions (50 μL protein sample plus 1 mL reagent) or 2500 microscale reactions (25 μL protein sample plus 200 μL reagent)
Kit Contents 500 mL BCA Solution, 15 mL 4% Cupric Sulfate, 3 x 1 mL BSA Standard, 2 mg/mL, Poly(dI-dC) solution, Sonicated salmon sperm DNA
Quantitation Range 20 to 2000 μg/mL
Quantity 1 Kit
Storage Requirements Store at room temperature