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Cytiva NHS-Activated Sepharose™ 4 Fast Flow Affinity Media

Catalog No. 45002965
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25 mL
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45002965 25 mL
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Catalog No. 45002965 Supplier Cytiva Supplier No. 17090601
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Description

Use for coupling amino-containing smaller proteins and peptides. Cytiva Life Sciences™ NHS-Activated Sepharose™ 4 Fast Flow Affinity Media yields coupled products that are very stable, especially at high pH.

  • Composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides
  • NHS (N-hydroxysuccinimide) coupling forms chemically stable amide bond with ligands containing primary amino groups
  • Resin provides spacer arm to coupled ligand
  • Fast Flow matrix gives high productivity
  • Coupled product is very stable, especially at high pH
  • Available in range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development

Specifications

Quantity 25 mL
For Use With (Application) For Coupling Amino-Containing Smaller Proteins and Peptides
Stability Stable in commonly used aqueous Buffers provided that the ligand withstand the pH or the chemical environment
Storage Requirements 2°C to 8°C
Product Type NHS-Activated Sepharose 4 Fast Flow Media
Flow Rate 150 to 250 cm/Hoμr
Particle Size 45 to 165 μm
Height (English) Bed 9.84 in.
Height (Metric) Bed 25 cm
Max. Pressure 100 kPa
Matrix 4% Cross-Linked Agarose
pH Range 3 to 13
Storage Buffer 100% Isopropanol
Ligand Type N-hydroxysuccinimide (NHS) Activated
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