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Invitrogen™ Neon™ Transfection System 100 μL Kit

Catalog No. p-7180351
Encompass
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No. of Reactions:
50 Reactions
96 x 2
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Catalog No. No. of Reactions
MPK10096 96 x 2
MPK10025 50 Reactions
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Catalog No. MPK10096 Supplier Invitrogen™ Supplier No. MPK10096
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Includes

30mL Resuspension Buffer R, 30mL Resuspension Buffer T, 2 x 150mL Electrolytic Buffer E2, 96 x 100μL Neon™ Tips, 20 Neon Electroporation Tubes

Neon™ Transfection System 100 μL Kit

  • For use with the Neon Transfection System
  • Use this kit for transfection volumes of 100μL, containing 5 x 105-2 x 106 adherent cells or 1 x 106-5 x 106 suspension cells
  • Cells that have been transfected using the included 100μL Neon Tips are then ready to be washed and plated into a 6-well, 60mm, or 10cm culture dish, depending on protocol

Adenoviral shRNA, Cell Culture, Lentiviral shRNA & miRNA, Plasmid Transfection, RNAi, RNAi Transfection, RNAi, Epigenetics & Noncoding RNA Research, Retroviral RNAi, Stem Cell & Primary Cell Transfections, Synthetic siRNA Transfection, Transfection, Vector-Based RNAi, siRNA

Order Info

Shipping Condition: Room Temperature

Specifications

Classification Animal Origin-Free, Chemically Defined, Low-Protein, Xeno-Free, Reduced Growth Factors, Reduced Serum
Content And Storage • 30 mL Resuspension Buffer R
• 30 mL Resuspension Buffer T
• 2 x 150 mL Electrolytic Buffer E2
• 96 x 100 μL Neon™ Tips
• 20 Neon™ Electroporation Tubes

Store the buffer box 4°C and the tips & tubes box at room temperature.
Cell Type Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample Type Plasmid DNA, Synthetic siRNA
Transfection Technique Electroporation
Concentration 5 x 10^5 - 2 x 10^6 cells/mL (Adherent Cells), 1 x 10^6 - 5 x 10^6 cells/mL
For Use With (Equipment) Neon™ Transfection System
For Use With (Application) Transfection
No. of Reactions 96 x 2
Product Line Neon
Product Type Transfection System Kit
Quantity 96 x 2 Reactions
Serum Compatible Yes
Shipping Condition Room Temperature
Starting Material Amount 2x10^4 - 6 x10^8 cells; Varies by cell type
System Type Neon™ Transfection System
Volume (Metric) Sample 100μl
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For Research Use Only. Not for use in diagnostic procedures.