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Invitrogen™ Neon™ Transfection System 100 μL Kit

Catalog No. mpk10025
Encompass
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No. of Reactions:
50 Reactions
96 x 2
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Catalog No. No. of Reactions
MPK10025 50 Reactions
MPK10096 96 x 2
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Catalog No. MPK10025 Supplier Invitrogen™ Supplier No. MPK10025
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Neon™ Transfection System 100 μL Kit

The Neon™ Transfection System 100 μL Kit is designed specifically for use with the MPK5000. Use this kit for transfection volumes of 100 μL, containing 5 × 105–2 × 106 adherent cells or 1 × 106–5 × 106 suspension cells. Cells that have been transfected using the included 100μL Neon™ Tips are then ready to be washed and plated into a 6-well, 60 mm, or 10 cm culture dish, depending on your protocol.

Specifications

Classification Animal Origin-Free, Chemically Defined, Low-Protein, Xeno-Free, Reduced Growth Factors, Reduced Serum
Content And Storage The Neon™ Transfection System 100 μL Kit comes with 10 mL resuspension buffer R, 10 mL resuspension buffer T, and 75 mL E2 electrolytic buffer in one box and 25 x 100μL reaction tips and 5 electroporation tubes in a separate box. Refer to the Neon™ Transfection System users manual for protocol instructions. Store the buffer box at 4°C and the tips & tubes box at room temperature.
Cell Type Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample Type Plasmid DNA, Synthetic siRNA
Promoter CMV
Transfection Technique Electroporation
For Use With (Equipment) Neon™ Transfection System
For Use With (Application) Transfection
No. of Reactions 50 Reactions
Product Line Neon
Product Type Transfection System
Quantity 25 x 2 Reactions
Serum Compatible Yes
Shipping Condition Room Temperature
Starting Material Amount 2x10^4 - 6 x10^8 cells; Varies by cell type
System Type Neon™ Transfection System
Volume (Metric) Sample 100μl
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For Research Use Only. Not for use in diagnostic procedures.