Invitrogen™ NCoR1 Polyclonal Antibody

Description

PA1-844A detects human and mouse nuclear receptor corepressor (N-CoR). PA1-844A has been successfully used in Western blot, ICC/IF, IHC (P) and immunoprecipitation (ChIP) procedures. By Western blot, this antibody detects a protein at ~270 kDa representing N-CoR from HeLa cell extracts and mouse RAW 264.7/primary macrophages. This antibody is not recommended for mouse C2C12 cells in immunofluorescent applications. The PA1-844A immunogen is a synthetic peptide corresponding to residues C P(2427) A P L L S A Q Y E T L S D S D D(2443) of mouse N-CoR. This immunizing peptide (Cat. # PEP-061) is available for use in neutralization and control experiments.

This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 17 and 20.