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Thermo Scientific™ N-PER™ Neuronal Protein Extraction Reagent

Catalog No. PI87792
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100 mL
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Catalog No. Quantity
PI87792 100 mL
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Catalog No. PI87792 Supplier Thermo Scientific™ Supplier No. LSG87792

Description

Collect proteins from all cellular compartments of neuronal tissue and primary cultured neurons with this proprietary, neuronal-specific cell lysis reagent.

Thermo Scientific™ N-PER Neuronal Protein Extraction Reagent is a proprietary cell lysis reagent optimized for efficient extraction of proteins from all cellular compartments of neuronal tissue and primary cultured neurons.

Neuron cell lysates prepared with the N-PER Reagent may be used for enzymatic activity assays (e.g., Phosphatase, Kinase, ATPase Assays), immunoassays (e.g., Western blots, ELISAs, RIAs) and protein purification. The N-PER Lysis Reagent enables protein extraction to be completed in less than 30 minutes and requires mechanical disruption of tissue (e.g., Dounce Homogenization, Polytron). Typical neuronal protein yields are 70-90μg of protein per mg of brain tissue or 300μg of total protein from 106 primary neuronal cells.

Highlights:

  • Optimized – efficient extraction of total neuronal protein, including membrane proteins, from tissue or primary cultured cells

  • Gentle – preserves protein function without compromising yield

  • Versatile – can be supplemented with protease inhibitors, reducing or chelating agents or required cofactors

  • Compatible – extracts are suitable for use with total protein, enzymatic and immunological assays and protein purification methods

Recommended for:

Isolate functional proteins from neuronal tissues or primary neuronal cells; Study enzymatic activity assays specific to neuronal tissue; Generate lysates from neuronal tissues for biomarker analysis

Specifications

Quantity 100 mL
Format Liquid
Product Type Neuronal Protein Extraction Reagent
Volume (Metric) 100 mL
Content And Storage Upon receipt store product at 4°C.
Product Line NE-PER

Frequently Asked Questions (FAQs)

Can you provide the shelf-life for the N-PER Neuronal Protein Extraction Reagent?

The N-PER Neuronal Protein Extraction Reagent is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
What lysis buffers do you offer for mammalian cells?

Please see the various lysis buffers we offer (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/cell-lysis-fractionation/cell-lysis-total-protein-extraction.html) for total protein extraction from mammalian cells.

For Research Use Only. Not for use in diagnostic procedures.