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MYLK (MLCK) Recombinant Human Protein DFS Item

MYLK (MLCK) Recombinant Human Protein

Manufacturer:  Life Technologies LSPV4339

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Catalog No. PV4339



Description

Description

MYLK (MLCK) is a serine⁄threonine kinase that phosphorylates myosin regulatory light chains to assist in the production of contractile activity (myosin-actin interaction).
Specifications

Specifications

NP_444253
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
67.3kDa
80% by SDS-PAGE
–80°C
1 Each
MYLK (MLCK)
MYLK (MLCK) was pre-diluted in enzyme dilution buffer and assayed in 10mM HEPES (pH 7.5), 10mM MgCl2, 1mM Na3VO4, 10μg/mL calmodulin, 0.5mM CaCl2, 2.5mM DTT, 200μM ATP, 200μg/mL ZIPtide peptide substrate (KKLNRTLSFAEPG) and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
MLCK
MYLK (MLCK) Recombinant Human Protein
Human
0.39mg/mL
4638
MYLK (MLCK) Recombinant Human Protein
10μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
MYLK (MLCK) Recombinant Human Protein
Kinase assay
Baculovirus
Amino acids 1425-1771, aa 1428-1771
76nmoles of phosphate transferred to ZIPtide peptide substrate (KKLNRTLSFAEPG) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 8.33μg/mL
GST Tag
Documents

For Research Use Only. Not for use in diagnostic procedures.