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Invitrogen™ MultiShot™ StripWell DH5α T1R Competent Cells

Catalog No. C449601
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1 x 96 tube rack (50 μl/tube)
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C449601 1 x 96 tube rack (50 μl/tube)
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Catalog No. C449601 Supplier Invitrogen™ Supplier No. C449601
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Description

Includes

Box 1:

  • MultiShot StripWell DH5α T1R Chemically Competent E. coli
  • One rack of 12 strips x 8-tubes, 50 μl per tube

Box 2:

  • pUC19 DNA (10 pg/μl): 1 vial, 50 μl
  • S.O.C. medium: 2 bottles, 15ml each

MultiShot StripWell DH5α T1R chemically competent E. coli cells are cloning competent cells packaged in a rack containing 12 strips of 8 tubes to increase productivity for medium-throughput bacterial transformations.

MultiShot StripWell DH5α T1R chemically competent E. coli cells are cloning competent cells packaged in a rack containing 12 strips of 8 tubes to increase productivity for medium-throughput bacterial transformations. This strain is derived from the popular DH5α strain, one of the most frequently used laboratory cloning strains. All of our T1R strains have the tonA (also known as fhuA) mutation in their genotype that confers resistance to T1 and T5 phages. A resistant genotype offers added security to protect valuable clones and libraries as T1 bacteriophage can rapidly spread among E. coli hosts and contaminate repositories.

DH5α T1R for routine cloning applications
The DH5α strain is well-known and broadly used laboratory strain for chemical transformations as it is ideal for transformations of cloned unmethylated DNA. To increase productivity for medium-throughput bacterial transformations, the cloning competent DH5α T1R E. coli cells are packaged in racks containing 12 strips of 8.

MultiShot StripWell DH5α T1R Competent Cells include:
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA
• tonA (fhuA) for T1 and T5 phage resistance 

MultiShot StripWell flexible format
Each StripWell of eight connected tubes is designed with the same single-tube, single-use features as our One Shot format tubes. This format allows all steps of the transformation protocol, up to plating, to take place in the same tube, saving time and reducing chance of contamination. After addition of DNA, MultiShot StripWell competent cells can be transformed by heat-shock at 42°C in a water bath without transfering to a new tube.

The StripWell tubes can also be cut to permit single transformations. This feature eliminates wasted aliquots and avoids needless freeze-thaw cycles that can result in reduced transformation efficiency. 

Benifits of the MultiShot StripWell format include:
• Increased productivity—suitable for high throughput experimentation 
• Maximal flexibility—transform as few as 1 tube or as many as 96 from the StripWell rack
• Familiar convenience—add DNA directly to cells, heat shock, and recover in the same tube

Genotype
F- φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ thi-1 gyrA96 relA1 tonA

Find the strain and format that you need
• We offer a variety of DH strains in both chemically competent and electrocompetent cell formats.
• For high-throughput transformation options, choose from our collection of MultiShot Formatted Competent Cells.

Specifications

Product Type Chemically Competent Cells
Contains F' Episome No
Cloning Methylated DNA No
Content And Storage • MultiShot StripWell DH5α T1R Competent Cells (1 x 96-well plate at 50 μL/well)
Store at –80°C.

• pUC19 DNA (50 μL at 10 pg/μL)
Store at –20°C.

• S.O.C. Medium (2 x 15 mL)
Store at 4°C or room temperature.
Antibiotic Resistance Bacterial No
Blue/White Screening Yes (lacZΔM15)
High-throughput Compatibility High
Preparing Unmethylated DNA No
Reduces Recombination Yes (recA1)
Shipping Condition Dry Ice
T1 Phage - Resistant (tonA) Yes
Species E. coli (K12)
Format 8-well Strips
Product Line Multishot
Quantity 1 x 96 tube rack (50 μl/tube)
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Frequently Asked Questions (FAQs)

Is it possible to perform a fast transformation protocol with the MultiShot competent cells?

There are situations where either because of time or process limitations, elimination of the heat shock and recovery time post-heat shock in the transformation protocol would be desirable. Testing was performed on many of our chemically competent cells in both the MultiShot StripWell and MultiShot FlexPlate format with a shortened transformation protocol. Use of a rapid transformation protocol (add DNA, no heat shock, no recovery period, plate on warm agar) yielded up to a log lower in transformation efficiency using the control DNA. This rapid transformation protocol can be performed with expectation of lower colony counts than our standard high efficiency transformation protocol and can only be performed with plasmids that use ampicillin as their antibiotic resistance. The rapid protocol is ideal when using a liquid handling system for high-throughput transformations with high cloning efficiency. Please see the product manual for full details on this rapid transformation protocol option.
Can I use a thermal cycler or heat block to heat shock the competent cells in the MultiShot FlexPlate?

The MultiShot FlexPlate is a PCR plate and can be placed in a thermal cycler or heat block set at 42 degrees C to transform the cells by the heat shock method. The duration of the heat shock performed in these devices has yielded similar transformation efficiencies as that seen with heat shock done in a water bath.
Which E. coli strains do you offer in the MultiShot StripWell format?

We offer the following E. coli strains in the MultiShot StripWell format: TOP10, DH5alpha T1R, Mach1 T1R, Stbl3, and BL21 Star (DE3) chemically competent cells.

Which E. coli strains do you offer in the MultiShot FlexPlate format?

We offer the following E. coli strains in the MultiShot FlexPlate format: TOP10, DH5alpha T1R, DH10B T1R, Mach1 T1R, OmniMAX2 T1R, and Stbl3 chemically competent cells.
What are the differences between the MultiShot StripWell, MultiShot FlexPlate, and MultiShot formats of E. coli cells?

The MultiShot StripWell format is supplied as pre-aliquoted competent cells in 96 tubes contained in a rack. The tubes are attached in strips of 8 tubes, and there are 12 strips that contain 50 µL of chemically competent cells. This format offers the most flexibility to transform 1 tube or up to all 96 tubes at once and is intended for varied or medium-throughput use.

The MultiShot FlexPlate format is supplied as pre-aliquoted competent cells in a 96-well PCR plate. Each well contains 20 µL of competent cells. The plate can be separated at each column of 8 wells, offering a high level of flexibility to utilize various increments of 8 wells per transformation. The wells of the plate are heat sealed, preventing detachment of the aluminum seal in the -80 degrees C freezer and preventing contamination. This format is compatible with automated liquid handling platforms and is intended for medium- to high-throughput use.

The MultiShot format is supplied as pre-aliquoted competent cells in 5, 96-well thin-wall polycarbonate microplates. Each well of the 96-well microplate contains 15 µL of chemically competent cells. This format is compatible with automated liquid handling platforms and is intended for high-throughput use.

For Research Use Only. Not for use in diagnostic procedures.