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Invitrogen™ MultiShot™ BL21(DE3) Chemically Competent E. coli Cells, 96-well plate

Catalog No. A40007051
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1 x 96 Well Plate (20 μL/Well)
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A40007051 1 x 96 Well Plate (20 μL/Well)
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Catalog No. A40007051 Supplier Invitrogen™ Supplier No. A40007051
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Description

MultiShot BL21(DE3) Chemically Competent E. coli Cells enable high-throughput protein expression with compatibility for automated liquid handling. This ready-to-use format streamlines cloning and expression workflows using the reliable BL21(DE3) expression strain.

Invitrogen MultiShot BL21(DE3) Chemically Competent E. coli Cells are suitable for the expression of non-toxic heterologous genes. They are pre-aliquoted into a 96-well PCR plate to increase transformation productivity.

Features of MultiShot BL21(DE3) competent cells include:

  • High transformation efficiency: >1x107 cfu/μg pUC19 DNA
  • Specifically constructed for high-level expression of recombinant proteins
  • Microtiter plate format design convenient for automated high-throughput cloning
  • Competent cells pre-aliquoted into 96-well plate, ready for transformation
  • S.O.C. Outgrowth medium included

MultiShot BL21(DE3) competent cells in a 96-well plate are designed to facilitate high-throughput transformation compatible with liquid handling equipment. The BL21 (DE3) strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG. BL21(DE3) is an E. coli B strain and does not contain the lon protease. It is also deficient in the outer membrane protease OmpT. The lack of these two key proteases reduces degradation of heterologous proteins expressed in the cells.

Genotype

F– ompT hsdSB (rB–, mB–) gal dcm (DE3)

Specifications

Product Type Chemically competent cells
Includes pUC19 DNA, S.O.C media
Transformation Efficiency Level ≥1.0 x 107
Shipping Condition Dry Ice
Species E. coli
Format 96-well Plate
Product Line MultiShot
For Use With (Application) Protein Expression
Quantity 1 x 96 Well Plate (20 μL/Well)

For Research Use Only.