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MP Biomedicals™ LymphoSep™ Lymphocyte Separation Medium

LymphoSep™ Lymphocyte Separation Medium is a sterile, iso-osmotic, low viscosity solution for density centrifugation yielding a clear separation of lymphocytes from heparinized or defibrinated human blood.

Manufacturer:  MP Biomedicals™ 091692254

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Catalog No. ICN1692254



  • Lymphocytes are isolated by layering diluted, heparinized blood over a solution of sodium metrizoate that contains a polysaccharide
  • Polysaccharide aggregates the erythrocytes so that they can be sedimented by low centrifugation
  • The less dense lymphocytes remain as a clearly defined band at the plasma Lymphosep interface
  • Provides:
    • In vitro isolation of lymphocytes from diluted whole blood
    • Maximum yield of mononuclear cells
    • Easy one-step centrifugation
    • >96% cell viability of lymphocytes
    • Low endotoxins
    • Autoclave sterilization


Lymphosep™ lymphocyte separation medium
6 to 9
15° to 30°C