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MP Biomedicals™ 0.25% Trypsin-EDTA (1X)

Catalog No. ICN1689649
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ICN1689649 100 mL
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Catalog No. ICN1689649 Supplier MP Biomedicals™ Supplier No. 091689649

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Description

0.25% Trypsin and 1mM EDTA in modified HBSS w/o Ca++ and Mg++, 100 mL

  • Trypsin-EDTA solutions are used to release adherent cells from culture surfaces.
  • Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues.
  • The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.
  • Ethylenediaminetetracetic acid (EDTA), a chelating agent, is added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cellto-cell adhesion and obscure the peptide bonds on which trypsin acts.

Specifications

Content And Storage -20°C
Concentration 1:250
Product Type Cell Culture Dissociation Reagent
Sterility Sterile
Without Additives No Calcium, No Magnesium
Formulation 0.25% (w/v) Solution, 0.25% Trypsin
pH 7 to 7.3
Quantity 100 mL
Source Porcine (for Trypsin)

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use.