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Invitrogen™ MOS MIP1 ALP CCL3 ELSA 10X96TS

Catalog No. 501125172
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10 x 96 Tests
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501125172 10 x 96 Tests
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Catalog No. 501125172 Supplier Invitrogen™ Supplier No. 885601388

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Description

ELISA

The Mouse Macrophage Inflammatory Protein 1 alpha (CCL3) (MIP-1α) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse MIP-1α. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.

Specifications

Accession Number P10855
Assay Range 0.8 to 50 pg/mL
Assay Sensitivity 0.8 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Mouse
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 20302
Gene Symbol AI323804,G0S19-1,LD78alpha,MIP-1 alpha,MIP1-(a),MIP1-alpha,Mip1a,Scya3
Kit Contents Capture Antibody: Pre-titrated, Purified antibody, Detection Antibody: Pre-titrated, Biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Synonym Small inducible cytokine A3, SCYA3, CCL3
Target CCL3 (MIP-1 alpha)
Test Time 1 hrs 45 mins
Total Assay Time 24 hrs 30 mins
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